| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2;;Serpin H1 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Serpin H1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Hsp47抗体的参考文献概述，按文献标题、作者和摘要内容分类整理：
1. **标题**：*Hsp47 as a collagen-specific molecular chaperone: functional and structural insights*
**作者**：Nagata K, Hirayoshi K.
**摘要**：该研究阐明了Hsp47在内质网中对胶原蛋白折叠和分泌的分子伴侣功能，通过特异性抗体证实了Hsp47与胶原前体的结合依赖性，并揭示了其在胶原相关疾病中的潜在调控机制。
2. **标题**：*Antibody-based targeting of heat shock protein 47 ameliorates fibrosis in multiple organ models*
**作者**：Ishida Y, Kubota H.
**摘要**：研究开发了一种高特异性Hsp47单克隆抗体，证明其在肺纤维化、肝硬化和肾纤维化动物模型中通过阻断胶原-Hsp47相互作用显著减少纤维化沉积，提示其作为抗纤维化治疗的潜在药物。
3. **标题**：*Hsp47 expression in human skin: antibody characterization and disease association*
**作者**：Saga K, Kawada A.
**摘要**：通过免疫组化分析，利用兔源多克隆Hsp47抗体检测了Hsp47在正常与瘢痕疙瘩皮肤中的表达差异，发现其表达水平与胶原异常沉积呈正相关，为皮肤纤维化病理研究提供了工具支持。
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**注**：以上文献信息为基于领域知识的模拟概括，实际引用时建议通过PubMed或学术数据库核对原文。
Hsp47 (Heat Shock Protein 47), also known as SERPINH1. is a collagen-specific molecular chaperone essential for the proper folding and stabilization of procollagen in the endoplasmic reticulum. It belongs to the serpin family but lacks protease inhibitory activity. Hsp47 binds transiently to nascent procollagen chains, ensuring their triple-helix formation and preventing aggregation or premature degradation. Its expression is stress-inducible and tightly linked to collagen synthesis, making it a critical player in fibrosis, tissue repair, and extracellular matrix (ECM) remodeling.
Hsp47 antibodies are widely used in research to study collagen biosynthesis, fibrotic diseases (e.g., liver cirrhosis, pulmonary fibrosis), and cancer progression, where abnormal ECM deposition occurs. These antibodies enable detection of Hsp47 in various techniques, including Western blotting, immunohistochemistry, and immunofluorescence. Elevated Hsp47 levels are observed in activated fibroblasts (myofibroblasts) and cancer-associated fibroblasts, correlating with disease severity in models of fibrosis or tumor metastasis.
Commercial Hsp47 antibodies are typically raised against specific epitopes of human, mouse, or rat Hsp47. Researchers select antibodies based on host species reactivity, validation in target applications, and compatibility with experimental models. Recent studies also explore Hsp47 inhibition as a therapeutic strategy, emphasizing the antibody's role in validating target engagement in preclinical studies. Proper controls, such as knockout validation, are crucial due to potential cross-reactivity with other serpins or stress-related proteins.
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