首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子白介素1(IL-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1A含量。IL1A即白介素-1α，是一种重要的细胞因子。其背景在于它在免疫调节、炎症反应中扮演关键角色。研究机制方面，它能激活下游信号通路，诱导炎症介质释放，参与多种疾病病理过程，如炎症性疾病、肿瘤等，对其研究有助于疾病治疗策略开发。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础研究中对多组样本进行批量检测，例如探索炎症模型动物血清标志物变化、评估药物干预后组织内细胞因子表达差异等场景，为实验室开展免疫调控机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：IL-1
Uniprot No.：P04822
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of rabbit IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 88-99 | ||||
| EDTA plasma (n=4) | 92 | 84-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7722.6412.7072.5475002.2162.0682.1421.9822501.2861.1861.2361.0761250.6520.6340.6430.48362.50.3700.3550.3630.20331.20.2820.2640.2730.11315.60.1990.2120.2060.04600.1640.1560.160 | ||||||
货期：3-5 working days
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