首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人锌指蛋白Aiolos(IKZF3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IKZF3含量。IKZF3 是重要靶点，它属于 IKAROS 转录因子家族。在正常生理中对淋巴细胞发育有调控作用。研究发现其异常表达与多种血液系统恶性肿瘤相关，如多发性骨髓瘤等，机制可能涉及对肿瘤细胞增殖、存活、凋亡等过程的影响。试剂盒检测范围为25 pg/mL-1600 pg/mL，本试剂盒采用高特异性配对抗体，可精准捕获目标蛋白，适用于科研领域对免疫调控机制、肿瘤微环境研究及药物干预效果评估等方向，为探索IKZF3在免疫应答、肿瘤免疫逃逸中的分子机制提供可靠工具。实验操作全程可在常规实验室条件下完成，兼容96孔板检测系统，支持批量样本分析，满足基础研究与转化医学中对生物标志物定量检测的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIO ELISA Kit; Aiolos ELISA Kit; IKAROS family zinc finger 3 (Aiolos) ELISA Kit; IKAROS family zinc finger 3 ELISA Kit; Ikaros family zinc finger protein 3 ELISA Kit; IKZF 3 ELISA Kit; IKZF3 ELISA Kit; IKZF3_HUMAN ELISA Kit; zinc finger DNA binding protein Aiolos ELISA Kit; Zinc finger protein Aiolos ELISA Kit; Zinc finger protein subfamily 1A 3 (Aiolos) ELISA Kit; Zinc finger protein subfamily 1A 3 ELISA Kit; Zinc finger protein subfamily 1A; member 3 ELISA Kit; ZNFN1A3 ELISA Kit
缩写：IKZF3
Uniprot No.：Q9UKT9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IKZF3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-109 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human IKZF3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.0981.9862.0421.9508001.3571.2981.3281.2364000.8250.7890.8070.7152000.5000.4810.4910.3991000.3600.3510.3560.264500.2450.2400.2430.151250.1560.1520.1540.06200.0920.0910.092 | ||||||
货期：3-5 working days
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