| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1;;p-MEK1 (S298) |
| WB Predicted band size | Calculated MW: 43 kDa ; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MEK1 around the phosphorylation site of S298 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于 **Phospho-MEK1(S298)** 抗体的3篇参考文献示例(文献信息为模拟，仅供参考)：
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1. **文献名称**: *"PAK1 phosphorylates MEK1 at Ser298 to regulate Raf/MEK/ERK signaling"*
**作者**: Frost JA et al.
**摘要**: 研究揭示了PAK1激酶通过磷酸化MEK1的S298位点，调控Raf/MEK/ERK信号通路的激活，并影响细胞增殖和迁移。实验中采用特异性Phospho-MEK1(S298)抗体验证了该位点的磷酸化依赖性。
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2. **文献名称**: *"Phosphorylation of MEK1 at Ser298 is required for TGF-β-induced epithelial-mesenchymal transition"*
**作者**: Zhang Y et al.
**摘要**: 本文发现TGF-β通过激活MEK1的S298磷酸化促进上皮-间质转化(EMT)，使用Phospho-MEK1(S298)抗体在乳腺癌细胞模型中证实了这一机制，表明该位点可能成为癌症治疗的潜在靶点。
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3. **文献名称**: *"MEK1 Ser298 phosphorylation modulates its kinase activity and substrate specificity"*
**作者**: Catalanotti F et al.
**摘要**: 通过体外激酶实验和Phospho-MEK1(S298)抗体的Western blot分析，发现S298磷酸化能增强MEK1对下游底物ERK的激活能力，并揭示其在MAPK信号反馈调控中的作用。
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如果需要具体文献，建议在 **PubMed** 或 **Google Scholar** 中搜索关键词 “Phospho-MEK1 Ser298 antibody” 或相关信号通路研究，或查阅抗体供应商(如CST、Abcam)提供的产品引用文献。
Phospho-MEK1(S298) antibodies are specialized tools used to detect the phosphorylation of MEK1 (mitogen-activated protein kinase kinase 1) at serine residue 298. a post-translational modification critical for regulating MEK1 activity and its role in cellular signaling. MEK1 is a central kinase in the MAPK/ERK pathway, which governs processes like cell proliferation, differentiation, and survival. Phosphorylation at S298. located in the proline-rich domain of MEK1. is thought to modulate interactions with upstream regulators (e.g., Raf kinases) or scaffold proteins, influencing subcellular localization or activation dynamics. This site is distinct from the canonical activation loop residues (S218/S222) phosphorylated during MEK1 activation, suggesting a regulatory role in signaling specificity or feedback mechanisms.
Phospho-MEK1(S298) antibodies enable researchers to study MEK1 regulation in response to stimuli (e.g., growth factors, stress) or pathological conditions (e.g., cancer, inflammation). They are widely used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess MEK1 activation states, pathway crosstalk, or drug effects. Validation of these antibodies typically includes verifying specificity using knockout cells or phosphorylation-blocking mutants. Dysregulation of MEK1 phosphorylation is implicated in diseases such as cancers with Ras/Raf mutations, making these antibodies valuable for both mechanistic studies and therapeutic development. Proper controls are essential to distinguish S298 phosphorylation from other MEK1 modifications.
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