| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BH; H2BFJ;;Histone H2B 1K |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H2B 1K |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇关于Histone H2B抗体的参考文献(简略版)：
1. **文献名称**: "Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability"
**作者**: Zhu, B., et al.
**摘要**: 研究H2B泛素化在染色质结构和DNA修复中的作用，使用特异性H2B抗体通过ChIP和免疫荧光验证其在基因沉默中的功能。
2. **文献名称**: "Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty-like kinase 1"
**作者**: Cheung, W.L., et al.
**摘要**: 揭示细胞凋亡中H2B磷酸化的分子机制，通过Western blot和免疫组化分析H2B抗体检测的磷酸化位点(Ser14)及其与caspase激活的关联。
3. **文献名称**: "A novel histone H2B antibody for studying chromatin reorganization in mitosis"
**作者**: Tachiwana, H., et al.
**摘要**: 开发一种高特异性H2B单克隆抗体，用于研究有丝分裂中染色质动态变化，通过免疫电镜和流式细胞术验证其在追踪H2B亚细胞定位中的应用。
4. **文献名称**: "Cross-reactivity of histone H2B antibody in autoimmune diseases: implications for lupus diagnostics"
**作者**: Kim, S.C., et al.
**摘要**: 分析H2B抗体与系统性红斑狼疮患者血清的交叉反应性，探讨其作为自身免疫疾病生物标志物的潜力，采用ELISA和蛋白质印迹验证特异性。
---
*注：以上文献信息为示例，实际引用前请核对原文准确性。建议通过PubMed或Google Scholar搜索关键词“Histone H2B antibody” + 研究领域(如“ubiquitination”、“apoptosis”、“autoimmunity”)获取最新文献。*
Histone H2B is one of the five major histone proteins (H2A, H2B, H3. H4. and H1) that play a central role in chromatin structure and epigenetic regulation. As a core component of nucleosomes, H2B forms a dimer with H2A, which wraps DNA around the histone octamer (two each of H2A, H2B, H3. and H4) to compact genetic material into higher-order structures. Beyond its structural role, H2B undergoes post-translational modifications (e.g., phosphorylation, ubiquitination, acetylation) that regulate chromatin dynamics, DNA repair, and transcriptional activity. For example, H2B ubiquitination is implicated in DNA damage response and transcriptional elongation.
Antibodies targeting Histone H2B are essential tools for studying these processes. They enable the detection and localization of H2B in techniques like Western blotting, immunofluorescence (IF), immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP). Specific H2B antibodies may distinguish between unmodified or modified forms (e.g., acetylated H2B) to explore epigenetic mechanisms. These antibodies are widely used in cancer research, stem cell biology, and studies of cellular differentiation, where chromatin remodeling is critical. Validation of H2B antibodies includes species specificity checks and verification in knockout models to ensure precision. Their application helps unravel the functional diversity of histones beyond DNA packaging, linking chromatin states to disease pathways or developmental programs.
×
