首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰岛素样生长因子结合蛋白2(IGFBP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGFBP2含量。IGFBP2（胰岛素样生长因子结合蛋白2）是一种高亲和力结合蛋白，在中枢神经系统的IGF（胰岛素样生长因子）调节中发挥重要作用。研究发现，IGFBP2在胶质瘤的发生、发展及预后中具有重要作用，其通过IGF依赖/非依赖通路、β-catenin通路等多种信号通路参与调节胶质瘤细胞的增殖、侵袭、转移及血管生成等生理过程。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研场景中探索IGFBP - 2在糖脂代谢调控、生长发育异常或肿瘤微环境中的作用机制，为研究IGF信号通路及相关疾病模型提供灵敏可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Igfbp2 ELISA Kit; Igfbp-2Insulin-like growth factor-binding protein 2 ELISA Kit; IBP-2 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP-2 ELISA Kit; BRL-BP ELISA Kit
缩写：IGFBP2
Uniprot No.：P12843
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 107 | |||||
| Range % | 95-111 | ||||||
| 1:40 | Average % | 104 | |||||
| Range % | 95-108 | ||||||
| 1:80 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
| 1:160 | Average % | 97 | |||||
| Range % | 87-103 | ||||||
回收率：
| The recovery of rat IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 82-94 | |||||
| EDTA plasma (n=4) | 101 | 90-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.6312.5682.6002.439202.0141.9641.9891.828101.1261.0831.1050.94450.5890.5670.5780.4172.50.3470.3560.3520.1911.250.2660.2770.2720.1110.6250.2190.2120.2160.05500.1590.1630.161 | |||||||
货期：3-5 working days
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