首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰岛素样生长因子结合蛋白1(IGFBP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IGFBP1含量。IGFBP1即胰岛素样生长因子结合蛋白1，它在调节细胞生长、分化和代谢等方面有重要作用。作为一种靶点，研究机制聚焦于其与胰岛素样生长因子的相互作用，以及对下游信号通路影响，以探寻其在肿瘤、代谢疾病等病理过程中的潜在干预方式。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研领域，可用于研究IGFBP-1在大鼠模型中的表达调控机制，探索其在代谢性疾病、生长发育异常或组织损伤修复中的潜在作用，亦可用于体外药物干预实验或疾病模型构建中相关生物标志物的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit
缩写：IGFBP1
Uniprot No.：P21743
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 80-94 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 88 | ||||
| Range % | 81-95 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 95-94 | |||||
回收率：
| The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.1422.2872.2152.030 5001.8821.8971.8901.705 2501.2971.3241.3111.126 1250.8030.8150.8090.624 62.50.4920.5060.4990.314 31.20.3600.3870.3740.189 15.60.2730.2880.2810.096 00.1840.1860.185 | ||||||
货期：3-5 working days
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