首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰岛素样生长因子2(IGF-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的IGF2含量。IGF2即胰岛素样生长因子 2，在胚胎发育和肿瘤发生中扮演重要角色。研究机制方面，它可与相应受体结合，激活下游信号通路，调控细胞的增殖、分化与存活。异常表达的 IGF2 与多种肿瘤的发生发展密切相关，是潜在的抗癌靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品为研究IGF - 2在肿瘤微环境中的作用机制、代谢相关疾病的动物模型评估、胚胎发育调控等基础科研领域提供可靠工具，尤其适用于需要多类型样本检测的体外实验研究，实验结果可通过酶标仪在450 nm波长下读取吸光度值进行分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C11orf43 ELISA Kit; IGF 2 ELISA Kit; IGF II ELISA Kit; IGF-II ELISA Kit; IGF2 ELISA Kit; IGF2_HUMAN ELISA Kit; IGFII ELISA Kit; INSIGF ELISA Kit; insulin like growth factor 2 (somatomedin A) ELISA Kit; Insulin like Growth Factor 2 ELISA Kit; Insulin like growth factor II ELISA Kit; Insulin like growth factor II precursor ELISA Kit; Insulin like growth factor type 2 ELISA Kit; pp9974 ELISA Kit; Preptin ELISA Kit; putative insulin like growth factor II associated protein ELISA Kit; Somatomedin A ELISA Kit; Somatomedin-A ELISA Kit
缩写：IGF2
Uniprot No.：P01344
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-104 | ||||
| EDTA plasma (n=4) | 97 | 90-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.7892.7692.7792.67320002.0342.0462.0401.93410001.5181.4781.4981.3925001.2051.2061.2061.1002500.8230.8390.8310.7251250.5360.5460.5410.43562.50.3490.3690.3590.25300.1020.1100.106 | ||||||
货期：3-5 working days
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