首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰岛素样生长因子1受体(IGF-1R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF1R含量。IGF1R（胰岛素样生长因子受体）是一种广泛表达于细胞膜的酪氨酸激酶受体，与细胞生长、存活和迁移密切相关。其研究机制涉及转录后调控，如m6A修饰和lncRNA调控，以及与肿瘤发生、心脏稳态等生理过程的关系。试剂盒检测范围为3.12 pg/mL-200 pg/mL，可支持科研人员在代谢调控机制探索、肿瘤微环境研究或组织再生实验中，通过高特异性定量分析IGF-1R的表达水平变化，其广泛的样本兼容性为跨组织、跨体液的多维度研究提供便利，特别适用于需要微量样本检测的高通量实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Igf1r; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD antigen CD221
缩写：IGF1R
Uniprot No.：P24062
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of rat IGF-1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 110 | 107-113 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 2002.4062.4612.4342.278 1001.9131.9461.9301.774 501.5261.5341.5301.374 251.0091.0681.0390.883 12.50.6840.6980.6910.535 6.250.3660.3680.3670.211 3.120.2570.2710.2640.108 00.1540.1570.156 | ||||||
货期：3-5 working days
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