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产品描述：人胰岛素样生长因子1受体(IGF-1R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF1R含量。人胰岛素样生长因子1受体（IGF-1R）是一种跨膜酪氨酸激酶受体，与IGF-1结合后调控细胞生长、增殖和分化。其在多种肿瘤中高表达，与肿瘤的发生发展密切相关。IGF-1R信号通路通过PI3K/AKT和Ras/MAPK等途径影响细胞周期、凋亡和侵袭。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础医学研究中IGF-1R信号通路的机制探索、肿瘤微环境研究、代谢性疾病模型构建以及靶向药物开发等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD221 ELISA Kit; CD221 antigen ELISA Kit; IGF 1 receptor ELISA Kit; IGF 1R ELISA Kit; IGF I receptor ELISA Kit; IGF-I receptor ELISA Kit; Igf1r ELISA Kit; IGF1R_HUMAN ELISA Kit; IGFIR ELISA Kit; IGFIRC ELISA Kit; IGFR ELISA Kit; Insulin like growth factor 1 receptor ELISA Kit; Insulin like growth factor 1 receptor precursor ELISA Kit; Insulin-like growth factor 1 receptor beta chain ELISA Kit; Insulin-like growth factor I receptor ELISA Kit; JTK13 ELISA Kit; MGC142170 ELISA Kit; MGC142172 ELISA Kit; MGC18216 ELISA Kit; Soluble IGF1R variant 1 ELISA Kit; Soluble IGF1R variant 2 ELISA Kit
缩写：IGF1R
Uniprot No.：P08069
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human IGF-1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 94-99 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.6342.7452.6902.544 101.7951.6871.7411.595 51.0991.1631.1310.985 2.50.7270.7450.7360.590 1.250.4460.4650.4560.310 0.6250.3310.3230.3270.181 0.3120.2650.2580.2620.116 00.1450.1460.146 | ||||||
货期：3-5 working days
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