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产品描述：人胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF-1含量。IGF-1即胰岛素样生长因子-1，在生长发育、细胞增殖等生理过程中起关键作用。其与多种疾病发生发展相关，如肿瘤。研究机制上，主要通过与受体结合激活下游信号通路，影响细胞生长、存活与代谢，是当前药物研发的重要靶点。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于体外培养模型、动物实验样本或临床前研究中的IGF - 1水平检测；可广泛应用于内分泌调控机制、组织再生研究、代谢性疾病机制探索等科研领域；满足基础医学、分子生物学等领域的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IBP1 ELISA Kit; IGF I ELISA Kit; IGF IA ELISA Kit; IGF IB ELISA Kit; IGF-I ELISA Kit; Igf1 ELISA Kit; IGF1_HUMAN ELISA Kit; IGF1A ELISA Kit; IGFI ELISA Kit; IGFIA ELISA Kit; Insulin like growth factor 1 (somatomedin C) ELISA Kit; Insulin like growth factor 1 ELISA Kit; Insulin like growth factor IA ELISA Kit; Insulin like growth factor IB ELISA Kit; Insulin-like growth factor I ELISA Kit; Mechano growth factor ELISA Kit; MGF ELISA Kit; OTTHUMP00000195080 ELISA Kit; OTTHUMP00000195081 ELISA Kit; OTTHUMP00000195082 ELISA Kit; OTTHUMP00000195083 ELISA Kit; OTTHUMP00000195084 ELISA Kit; Somatomedia C ELISA Kit; Somatomedin C ELISA Kit; Somatomedin-C ELISA Kit
缩写：IGF-1
Uniprot No.：P05019
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-101 | |||||
回收率：
| The recovery of human IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 91-95 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 5002.7852.7522.7692.627 2502.2192.1892.2042.062 1251.3881.2981.3431.201 62.50.7020.7240.7130.571 31.20.4340.4460.4400.298 15.60.3070.3150.3110.169 7.80.2280.2160.2220.080 00.1430.1410.142 | ||||||
货期：3-5 working days
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