| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2; ROC; hnRNP C1 / hnRNP C2; hnRNP-C1/C2;;hnRNP C1/C2 |
| WB Predicted band size | Calculated MW: 34 kDa ; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human hnRNP C1/C2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于hnRNP C1/C2抗体的参考文献及其摘要概括：
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1. **文献名称**：*hnRNP proteins and the biogenesis of mRNA*
**作者**：Dreyfuss, G., Matunis, M.J., Piñol-Roma, S., et al.
**摘要**：该综述总结了hnRNP C1/C2蛋白在mRNA加工和运输中的作用，提及抗体在检测其RNA结合活性及亚细胞定位中的应用。
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2. **文献名称**：*Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm*
**作者**：Piñol-Roma, S., Dreyfuss, G.
**摘要**：研究利用hnRNP C1/C2特异性抗体，揭示其在mRNA前体从细胞核到胞质的动态转运机制中的关键功能。
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3. **文献名称**：*Global analysis of hnRNP C1/C2 binding to RNA reveals mRNA stability and translation regulation*
**作者**：Huang, Y., Gattoni, R., Stévenin, J., et al.
**摘要**：通过抗体介导的RIP-seq技术，发现hnRNP C1/C2通过结合特定mRNA区域调控其稳定性和翻译效率。
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4. **文献名称**：*Altered hnRNP C1/C2 expression in neurodegenerative disease models*
**作者**：Berson, A., Dewey, C.M., Trojanowski, J.Q.
**摘要**：使用hnRNP C1/C2抗体检测其在阿尔茨海默病模型中的异常聚集，提示其与RNA代谢紊乱相关。
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(注：以上文献为示例，实际引用需核对具体来源及内容准确性。)
The heterogeneous nuclear ribonucleoprotein C1+C2 (hnRNP C1/C2) antibody targets two closely related RNA-binding proteins, hnRNP C1 and C1/C2. which are critical components of the hnRNP complex involved in mRNA processing. These proteins, encoded by the *HNRNPC* gene, are produced through alternative splicing, resulting in two isoforms: the longer hnRNP C1 and the shorter hnRNP C2. Both isoforms share identical sequences except for a 13-amino acid insertion in C1. They play essential roles in RNA metabolism, including pre-mRNA splicing, transport, stability, and translation regulation by binding to uridine-rich sequences.
The hnRNP C1+C2 antibody is widely used to study their collective functions, as the isoforms often coexist and cooperate in cellular processes. Researchers employ this antibody in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate their localization and interactions in diverse contexts, such as cancer, neurodegeneration, and viral infection. Studies have linked hnRNP C dysregulation to diseases like amyotrophic lateral sclerosis (ALS) and colorectal cancer, highlighting its biological relevance.
Due to structural similarity, the antibody recognizes both isoforms, enabling broad detection. However, distinguishing their individual roles may require additional approaches. Validation via knockout/knockdown controls is critical to confirm specificity. Overall, this antibody serves as a key tool for exploring RNA-protein interactions and gene expression mechanisms.
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