首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠免疫球蛋白A(IgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgA含量。IgA作为靶点有重要意义。IgA是人体黏膜免疫的主要抗体。研究机制上，围绕其与病原体相互作用、在黏膜表面防御机制等开展，旨在通过干预IgA相关通路，研发治疗感染性疾病、自身免疫病等的药物，为攻克相关病症提供新策略。试剂盒检测范围为7.81 ng/mL-2000 ng/mL，本产品适用于免疫学研究、感染性疾病模型建立、疫苗开发评估等科研领域，特别适用于长期监测实验动物体液免疫应答动态，或探究免疫调节剂、营养干预等外界因素对大鼠IgA分泌的影响，为免疫相关机制研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：7.81 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:2000 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4000 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:8000 | Average % | 103 | ||||
| Range % | 99-107 | |||||
回收率：
| The recovery of rat IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 20000.2380.2310.235 5000.4470.4240.436 1250.6920.6570.675 31.251.2241.2851.255 7.811.7981.7371.768 02.0632.0452.054 | ||||||
货期：3-5 working days
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