| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PP2A alpha; PP2A beta; Replication protein C; PPP2CA; PPP2CB;;PP2A alpha/beta |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PP2A alpha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PP2Aα/β抗体的3篇参考文献的简要总结：
1. **"Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling"**
*作者：Virshup, D. M.*
摘要：该综述探讨了PP2A的结构与功能，强调其α和β亚基在不同组织中的分布差异，并讨论了特异性抗体在亚基定位和功能研究中的应用，为理解PP2A在细胞信号传导中的作用提供依据。
2. **"PP2A: the tumor suppressor that controls retinoic acid-induced transcript repression and senescence"**
*作者：Janssens, V., Goris, J.*
摘要：研究通过PP2Aα/β抗体分析亚基表达，发现PP2Aα在维持细胞稳态中起关键作用，而β亚基可能参与分化调控，抗体检测显示其在癌症中表达异常，与肿瘤抑制功能相关。
3. **"Dysregulation of Protein Phosphatase 2A in Alzheimer’s Disease: Role in Tau Hyperphosphorylation"**
*作者：Sontag, E., et al.*
摘要：利用PP2Aα/β特异性抗体，研究发现阿尔茨海默病患者脑组织中PP2A活性降低，α亚基表达下降导致tau蛋白过度磷酸化，提示PP2A功能障碍与神经退行性疾病相关。
(注：以上文献信息为示例，实际引用需根据具体研究核实作者、标题及摘要内容。)
The PP2Aalpha+beta antibody is designed to detect both the alpha (PR65α) and beta (PR65β) isoforms of the structural A subunit of Protein Phosphatase 2A (PP2A), a critical serine/threonine phosphatase involved in diverse cellular processes. PP2A exists as a heterotrimeric holoenzyme composed of a scaffolding A subunit, a catalytic C subunit, and a regulatory B subunit. The A subunit serves as a structural platform, with two major isoforms (alpha and beta) encoded by separate genes (PPP2R1A and PPP2R1B). While these isoforms share ~85% sequence homology, they exhibit distinct expression patterns: PP2Aα is ubiquitously expressed, whereas PP2Aβ shows tissue-specific localization, particularly in the brain and heart.
Antibodies targeting both isoforms enable researchers to study PP2A’s roles in cell cycle regulation, signal transduction, apoptosis, and metabolism without distinguishing between the two A subunit variants. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation to assess PP2A expression, localization, and protein interactions. Dysregulation of PP2A, including altered A subunit expression, has been implicated in cancer, neurodegenerative diseases (e.g., Alzheimer’s), and cardiovascular disorders. By detecting total A subunit levels, the PP2Aalpha+beta antibody aids in exploring disease mechanisms or therapeutic responses linked to PP2A dysfunction. Its broad applicability makes it a valuable tool for investigating PP2A’s complex biology across physiological and pathological contexts.
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