| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TPH1;MGC119994;TPRH;TRPH;Tryptophan 5-hydroxylase 1; Tryptophan Hydroxylase;;TPH1 |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TPH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于 **TPH1抗体** 的参考文献示例(内容为假设性概括，建议通过学术数据库验证原文)：
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1. **"Development and Validation of a Specific Antibody for Tryptophan Hydroxylase 1 (TPH1) in Peripheral Tissues"**
*Authors: Smith A, et al. (2020)*
**摘要**：本研究开发了一种高特异性TPH1抗体，用于区分TPH1与TPH2的同源结构。通过免疫印迹和免疫组化验证，证明该抗体可准确检测肠道和肝脏中的TPH1表达，为外周血清素合成研究提供可靠工具。
2. **"TPH1 Expression in Carcinoid Tumors: A Comparative Study Using Novel Antibody Markers"**
*Authors: Lee B, et al. (2018)*
**摘要**：利用新型TPH1抗体分析类癌肿瘤中色氨酸羟化酶的分布，发现TPH1在肠嗜铬细胞中高表达，并与血清素分泌水平相关，提示其作为肿瘤标志物的潜力。
3. **"Role of TPH1 in Hepatic Fibrosis: Insights from Antibody-Based Knockdown Models"**
*Authors: García-Ruiz C, et al. (2016)*
**摘要**：通过TPH1抗体介导的蛋白敲低实验，揭示TPH1在肝星状细胞活化中的关键作用，表明抑制TPH1可减轻纤维化进程，为肝病治疗提供新靶点。
4. **"Cross-Reactivity Challenges in TPH1/TPH2 Antibodies: A Methodological Review"**
*Authors: Patel R, et al. (2015)*
**摘要**：系统性评估市售TPH1抗体的特异性，指出部分抗体存在与TPH2的交叉反应，强调使用基因敲除模型验证抗体特异性的必要性。
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**注意**：以上文献信息为示例，实际引用请通过 **PubMed、Web of Science 或 Google Scholar** 检索真实文献。建议结合关键词“TPH1 antibody”、“validation”或“immunohistochemistry”进一步筛选。
The tryptophan hydroxylase 1 (TPH1) antibody is a crucial tool for studying the enzyme TPH1. which catalyzes the rate-limiting step in serotonin synthesis. TPH1 is one of two isoforms (TPH1 and TPH2) of tryptophan hydroxylase, with TPH1 primarily expressed in peripheral tissues, such as the gut enterochromaffin cells, pineal gland, and peripheral neurons, while TPH2 is localized to the central nervous system. TPH1 converts tryptophan to 5-hydroxytryptophan (5-HTP), which is subsequently decarboxylated to serotonin (5-HT). Serotonin plays vital roles in gastrointestinal function, mood regulation, and cardiovascular homeostasis, linking TPH1 to disorders like irritable bowel syndrome, depression, and carcinoid tumors.
TPH1 antibodies are widely used in research to detect and quantify TPH1 protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate TPH1's expression patterns, regulatory mechanisms, and its dysregulation in diseases. For example, TPH1 upregulation is observed in neuroendocrine tumors, while its inhibition is explored as a therapeutic strategy for conditions involving excessive serotonin production, such as carcinoid syndrome. Commercial TPH1 antibodies are typically validated for specificity against TPH1. distinguishing it from TPH2 due to structural differences.
Research on TPH1 antibodies also supports drug development, including TPH1 inhibitors like telotristat ethyl, approved to manage carcinoid syndrome diarrhea. Overall, TPH1 antibodies are indispensable for advancing understanding of serotonin biology and related pathologies.
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