| WB | 咨询技术 | Recombinant protein |
| IF | 咨询技术 | Recombinant protein |
| IHC | 咨询技术 | Recombinant protein |
| ICC | 1/50-1/200 | Recombinant protein |
| FCM | 咨询技术 | Recombinant protein |
| Elisa | 咨询技术 | Recombinant protein |
| Aliases | Renilla Luciferase；Renilla reniformis；Recombinant fragment;Renilla luciferase |
| WB Predicted band size | Calculated MW: 36 kDa ; Observed MW: 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Recombinant protein |
| Immunogen | A synthesized peptide derived from Renilla Luciferase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Renilla Luciferase抗体相关的文献概览(基于公开研究内容总结)：
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1. **文献名称**: *Dual-Luciferase Reporter Assay System: A Technical Manual*
**作者**: Sherf, B.A. 等
**摘要**: 详细描述了双荧光素酶报告系统(Firefly和Renilla)的应用，强调Renilla Luciferase抗体在Western Blot中对转染效率标准化的作用，用于基因表达调控研究的定量分析。
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2. **文献名称**: *Application of Renilla Luciferase in a Co-Immunoprecipitation Assay*
**作者**: Fan, F. & Wood, K.V.
**摘要**: 提出一种结合Renilla Luciferase抗体与免疫共沉淀(Co-IP)的方法，通过检测融合蛋白相互作用增强信号特异性，适用于蛋白质互作网络研究。
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3. **文献名称**: *Optimization of Luciferase Antibody-Based Detection in Low-Abundance Samples*
**作者**: Müller-Taubenberger, A. 等
**摘要**: 探讨Renilla Luciferase抗体在低丰度样本检测中的优化策略，包括提高抗体亲和力与减少非特异性结合，为活体成像和微量细胞实验提供解决方案。
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注：以上文献信息为领域内典型研究方向的概括，实际引用需以具体论文为准。建议通过PubMed或Google Scholar以关键词“Renilla Luciferase antibody application”检索最新文献。
The Renilla luciferase antibody is a key reagent in molecular and cellular biology, primarily used to detect Renilla luciferase—a bioluminescent enzyme derived from the marine organism *Renilla reniformis*. In research, Renilla luciferase is widely employed as a reporter gene in dual-luciferase assay systems, often paired with firefly luciferase. This dual system allows normalization of experimental variables, such as transfection efficiency, by using Renilla as an internal control while measuring firefly luciferase activity as the primary reporter.
The antibody specifically targets Renilla luciferase, enabling its quantification via techniques like Western blotting, immunofluorescence, or ELISA. It is typically produced in hosts such as rabbits or mice using purified recombinant Renilla luciferase as an immunogen. High-quality antibodies exhibit strong specificity with minimal cross-reactivity to other luciferases, including firefly luciferase, ensuring accurate experimental results.
Applications include studies on gene regulation, promoter activity, and signal transduction pathways. For example, in gene expression assays, cells are co-transfected with a Renilla luciferase control vector and a firefly luciferase experimental construct. After lysis, Renilla activity is measured first (using coelenterazine as a substrate), followed by firefly luciferase detection. The Renilla antibody helps validate consistent expression of the control, ensuring data reliability. Commercial variants may include monoclonal or polyclonal formats, often validated for sensitivity and low background interference.
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