| WB | 咨询技术 | Recombinant protein |
| IF | 咨询技术 | Recombinant protein |
| IHC | 咨询技术 | Recombinant protein |
| ICC | 1/50-1/200 | Recombinant protein |
| FCM | 咨询技术 | Recombinant protein |
| Elisa | 咨询技术 | Recombinant protein |
| Aliases | Luciferase; Firefly; Luciferin 4 monooxygenase;;Firefly luciferase |
| WB Predicted band size | Calculated MW: 61 kDa ; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Recombinant protein |
| Immunogen | A synthesized peptide derived from Firefly luciferase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Firefly Luciferase抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*"Dual-luciferase reporter assay: an advanced co-reporter system integrating Firefly and Renilla luciferase detection"*
**作者**：SDS Laboratories et al.
**摘要**：该文献提出双荧光素酶报告系统优化方案，强调使用Firefly Luciferase抗体(如抗-FLuc单克隆抗体)选择性抑制Firefly活性，避免与海肾荧光素酶(Renilla)交叉反应，从而提高定量准确性。
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2. **文献名称**：*"Characterization of a monoclonal antibody specific to Firefly Luciferase for mammalian reporter gene assays"*
**作者**：Hsu CY, et al.
**摘要**：研究团队开发了一种高特异性单克隆抗体，可识别Firefly Luciferase的C端结构域。通过Western blot和免疫沉淀验证其灵敏度(检测限0.1 ng)，并成功用于活细胞成像中荧光素酶融合蛋白的追踪。
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3. **文献名称**：*"Application of Firefly Luciferase antibody in studying protein-protein interactions via NanoBiT complementation"*
**作者**：Fan F, Wood KV.
**摘要**：文献将Firefly Luciferase抗体应用于基于NanoLuc的互补检测系统，通过抗体介导的荧光素酶构象稳定化，增强分裂式报告蛋白(如HiBiT/LgBiT)的组装效率，提升低丰度靶标的检测灵敏度。
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**备注**：以上文献名为虚拟示例，实际研究中可检索PubMed或抗体供应商(如Thermo Fisher、Abcam)提供的产品引用文献，关键词为“Firefly Luciferase antibody application”或“anti-FLuc antibody validation”。
Firefly luciferase (FLuc) antibody is a crucial tool in molecular and cellular biology, primarily used to detect and quantify the presence of firefly luciferase, an enzyme derived from the North American firefly (*Photinus pyralis*). FLuc catalyzes the oxidation of D-luciferin in the presence of ATP and oxygen, producing light (bioluminescence) that serves as a sensitive reporter in various assays. FLuc antibodies are widely employed in techniques like Western blotting, ELISA, immunoprecipitation, and immunofluorescence to monitor FLuc-tagged proteins, gene expression patterns, or transgenic models.
These antibodies are typically raised against purified FLuc protein or specific epitopes, available as monoclonal or polyclonal formats. Monoclonal antibodies offer high specificity, ideal for distinguishing FLuc from other luciferases (e.g., Renilla luciferase) in dual-reporter systems, while polyclonal antibodies may provide higher sensitivity due to multiple epitope recognition. FLuc antibodies are critical in drug discovery, promoter activity studies, and protein trafficking research, where precise quantification of reporter activity is essential. Validation often involves testing against FLuc-expressing cell lysates or recombinant proteins to confirm specificity and absence of cross-reactivity. Their reliability ensures accurate normalization and interpretation in experiments leveraging FLuc-based bioluminescence imaging or high-throughput screening.
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