| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C myb; Myb proto oncogene; v Myb; Myb protein;;p-c Myb (S11) |
| WB Predicted band size | Calculated MW: 72 kDa ; Observed MW: 72,80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human c Myb around the phosphorylation site of S11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Myb抗体的示例参考文献(内容为模拟示例，实际文献请通过学术数据库查询)：
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1. **标题**: *c-Myb Antibody Characterization in Leukemia Cell Lines*
**作者**: Smith J, et al.
**摘要**: 研究验证了一种特异性识别人类c-Myb蛋白的单克隆抗体，通过Western blot和免疫荧光技术确认其在急性髓系白血病细胞系中的高表达，为白血病机制研究提供工具。
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2. **标题**: *Role of Myb in Colorectal Cancer Progression: An Immunohistochemical Study*
**作者**: Lee H, et al.
**摘要**: 利用Myb抗体对结直肠癌组织进行免疫组化分析，发现Myb蛋白过表达与肿瘤侵袭性和不良预后显著相关，提示其作为潜在生物标志物的价值。
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3. **标题**: *Development of a Polyclonal Antibody Against Avian Myb for Developmental Biology*
**作者**: Garcia R, et al.
**摘要**: 报道了一种针对禽类Myb蛋白的多克隆抗体制备方法，并验证其在胚胎发育组织中特异性检测Myb的表达模式，支持其在发育调控研究中的应用。
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4. **标题**: *Myb Oncoprotein Inhibition in Triple-Negative Breast Cancer Models*
**作者**: Chen L, et al.
**摘要**: 通过Myb抗体阻断实验，证明Myb蛋白在三阴性乳腺癌细胞增殖中起关键作用，为靶向治疗提供实验依据。
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建议通过PubMed、Google Scholar等平台检索最新文献(关键词：Myb antibody, c-Myb detection)以获取真实数据。
Myb antibodies are immunological tools targeting proteins encoded by the MYB gene family, which includes key transcription factors like c-Myb, A-Myb, and B-Myb. These proteins play pivotal roles in regulating cellular processes such as proliferation, differentiation, and apoptosis by binding to specific DNA sequences to activate or repress target genes. c-Myb, the most studied member, is critical in hematopoiesis and stem cell maintenance, while A-Myb and B-Myb are implicated in tissue-specific functions, including spermatogenesis and cell cycle regulation.
Myb antibodies are widely used in research to detect and quantify MYB protein expression, aiding studies on their physiological and pathological roles. Dysregulation of MYB proteins is linked to cancers, particularly hematologic malignancies (e.g., leukemia) and solid tumors (e.g., breast and colorectal cancers). Overexpression or mutations in MYB genes may drive oncogenesis, making these proteins potential therapeutic targets.
These antibodies are essential in techniques like Western blotting, immunohistochemistry, and flow cytometry. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple epitopes. Validation is crucial to ensure reliability, as off-target binding can skew results. Researchers also use Myb antibodies in functional assays (e.g., ChIP-seq) to map DNA-binding sites and study gene regulatory networks. Their application extends to drug development, where MYB inhibition is explored as a strategy to curb cancer progression.
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