| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATP-binding cassette transporter 8; White protein homolog; ABC8; WHT1;ABCG1; ABC transporter 8; WHITE1;;ABCG1 |
| WB Predicted band size | Calculated MW: 76 kDa ; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human ABCG1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ABCG1抗体的3篇代表性文献及其摘要内容：
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1. **文献名称**：*ABCA1 and ABCG1 synergize to maintain cholesterol homeostasis in human macrophages*
**作者**：Baldán Á, et al.
**摘要**：该研究利用ABCG1抗体检测巨噬细胞中ABCG1蛋白的表达水平，发现ABCA1和ABCG1在调控细胞胆固醇外流中具有协同作用，其缺失导致脂质蓄积，提示二者在动脉粥样硬化中的潜在关联。
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2. **文献名称**：*Loss of ABCG1 leads to altered cholesterol trafficking and increased amyloidogenesis in the brain*
**作者**：Wojcik AJ, et al.
**摘要**：通过ABCG1抗体进行免疫组化分析，发现ABCG1敲除小鼠脑组织中胆固醇转运异常，β-淀粉样蛋白沉积增加，提示ABCG1在阿尔茨海默病病理中的保护作用。
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3. **文献名称**：*Dynamic localization of ABCG1 in intracellular and plasma membrane pools in hepatocytes*
**作者**：Tarling EJ, et al.
**摘要**：研究利用ABCG1抗体进行亚细胞定位分析，发现肝细胞中ABCG1在脂质负荷下从内质网向质膜转位，参与胆汁酸分泌调节，为肝脏脂代谢机制提供新见解。
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4. **文献名称**：*ABCG1 regulates PDGF-induced proliferation and migration of vascular smooth muscle cells*
**作者**：Kobayashi A, et al.
**摘要**：采用ABCG1抗体通过Western blot和免疫荧光技术，揭示ABCG1通过调控胆固醇外流抑制PDGF信号通路，从而影响血管平滑肌细胞增殖，提示其在血管疾病中的治疗潜力。
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这些文献展示了ABCG1抗体在胆固醇代谢、神经退行性疾病、肝脏功能及血管病理中的关键应用。如需具体实验细节或扩展文献，可进一步补充。
ABCG1 (ATP-binding cassette sub-family G member 1) is a transmembrane protein belonging to the ATP-binding cassette (ABC) transporter superfamily, which plays critical roles in lipid homeostasis. Primarily expressed in macrophages, liver, and endothelial cells, ABCG1 facilitates cholesterol efflux to high-density lipoprotein (HDL), thereby regulating cellular cholesterol balance and preventing lipid accumulation. Its function is closely associated with atherosclerosis, as defective cholesterol transport contributes to foam cell formation in arterial walls. ABCG1 also participates in immune modulation and may influence diseases like diabetes and neurodegenerative disorders.
ABCG1 antibodies are essential tools for investigating its expression, localization, and mechanistic roles in lipid metabolism and disease. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to detect ABCG1 in tissues or cultured cells. Researchers employ them to study ABCG1's interaction with lipid carriers, its regulation under pathological conditions (e.g., oxidative stress or inflammation), and its potential as a therapeutic target. Validation of antibody specificity often involves knockout controls or siRNA-mediated gene silencing. Recent studies also explore ABCG1's role in cancer drug resistance due to its efflux capabilities. Commercial ABCG1 antibodies are typically raised against specific epitopes, such as the N-terminal or cytoplasmic domains, with variations in host species (e.g., rabbit, mouse) and conjugates (e.g., HRP, fluorescent tags) to suit experimental needs.
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