| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cubilin; Cubn; IFCR; MGA1; Cubilin precursor; megaloblastic anemia 1;;Cubilin |
| WB Predicted band size | 399 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cubilin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Cubilin抗体的参考文献及其摘要概括：
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1. **文献名称**: *The intrinsic factor-vitamin B12 receptor and target of teratogenic antibodies is a novel protein complex in the kidney*
**作者**: Moestrup, S.K. et al.
**摘要**: 该研究首次鉴定了Cubilin作为内因子-维生素B12复合物的受体蛋白，并证实其与肾近端小管的重吸收功能相关。通过特异性抗体，揭示了Cubilin在肾脏和小肠中的定位，及其在介导维生素B12吸收中的关键作用。
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2. **文献名称**: *Cubilin dysfunction causes abnormal metabolism of vitamin B12 in the kidney*
**作者**: Fyfe, J.C. et al.
**摘要**: 研究利用Cubilin特异性抗体，分析了Imerslund-Gräsbeck综合征患者的肾组织样本，发现Cubilin表达缺失导致维生素B12代谢异常，证实了Cubilin基因突变与该遗传性疾病的相关性。
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3. **文献名称**: *Cubilin is essential for albumin reabsorption in the renal proximal tubule*
**作者**: Birn, H. et al.
**摘要**: 通过免疫荧光和Western blot技术(使用Cubilin抗体)，发现Cubilin在肾近端小管中与megalin协同介导白蛋白的重吸收。抗体阻断实验进一步证明其功能缺失会导致蛋白尿。
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这些研究均利用Cubilin抗体揭示了其在维生素吸收、遗传性疾病及肾脏功能中的关键机制。
**Background of Cubilin Antibodies**
Cubilin, also known as the intrinsic factor-cobalamin receptor, is a multiligand endocytic receptor protein primarily expressed in polarized epithelial cells of the kidney, intestine, and yolk sac. It plays a critical role in nutrient absorption, particularly vitamin B12 (cobalamin) in the small intestine and protein reabsorption in the kidney proximal tubule. Structurally, cubilin lacks transmembrane domains and relies on interaction with megalin (LRP2) for cell surface anchorage and endocytic trafficking. Its large extracellular region contains multiple CUB domains, which mediate ligand binding to diverse molecules, including albumin, transferrin, and apolipoproteins.
Cubilin dysfunction is linked to genetic disorders like Imerslund-Gräsbeck syndrome (characterized by vitamin B12 deficiency and proteinuria) and acquired conditions such as chronic kidney disease. Research on cubilin antibodies has been pivotal in elucidating its physiological roles and pathological implications. These antibodies, often targeting specific CUB domains or epitopes, are utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study cubilin expression, localization, and interaction partners.
Recent studies also explore cubilin’s involvement in renal pathologies (e.g., diabetic nephropathy, IgA nephropathy) and cancer, where altered cubilin expression may influence disease progression. Therapeutic strategies targeting cubilin pathways, including antibody-based interventions, are under investigation for treating malabsorption syndromes or kidney disorders. Cubilin antibodies thus serve as essential tools for both basic research and clinical applications.
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