| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Mouse anti Rat IgG2a-HRP，Mouse anti Rat IgG2a HRP |
| Conjugation | HRP |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Secondary Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Rat IgG2a |
| Formulation | Purified antibody in PBS with 0.01% thimerosal. |
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以下是3条关于Rat IgG2a-HRP抗体的示例参考文献(内容为模拟概括，仅供参考)：
1. **《Optimization of HRP-Conjugated Secondary Antibodies for Immunoblotting》**
- 作者：Smith, K.J. et al.
- 摘要：研究比较了多种HRP标记的二抗(包括Rat IgG2a-HRP)在Western Blot中的灵敏度，验证其在低丰度蛋白检测中的高特异性，并优化了封闭缓冲液和稀释条件以减少非特异性结合。
2. **《Characterization of Cross-Reactivity in Commercial HRP-Labeled Antibodies》**
- 作者：Johnson, R. & Lee, M.
- 摘要：通过ELISA和免疫组化实验评估Rat IgG2a-HRP抗体的交叉反应性，证明其对大鼠IgG2a亚型的高度选择性，且与其他物种(小鼠、兔)IgG无显著交叉反应，适用于多标实验。
3. **《Applications of HRP-Based Detection in Multiplex Immunoassays》**
- 作者：Chen, H. et al.
- 摘要：探讨Rat IgG2a-HRP抗体在流式细胞术和免疫荧光中的兼容性，提出其在多色检测系统中与荧光标记抗体联用的方案，并强调化学发光底物选择对信号强度的影响。
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**注**：以上为示例文献，实际文献需通过PubMed/Google Scholar等平台检索关键词(如"Rat IgG2a-HRP antibody validation")。推荐查阅抗体供应商提供的技术手册或应用案例(如Thermo Fisher、Abcam)。
Rat IgG2a-HRP is a secondary antibody conjugated to horseradish peroxidase (HRP), commonly used in immunoassays to detect primary antibodies of the rat IgG2a subclass. Rat IgG2a, an immunoglobulin subclass, plays a role in adaptive immune responses, particularly in opsonization and complement activation. The HRP conjugation enables enzymatic signal amplification in detection methods like Western blotting, ELISA, or immunohistochemistry.
This antibody is typically produced in a host species (e.g., mouse, goat, or rabbit) immunized with rat IgG2a. It specifically binds to the Fc region of rat IgG2a antibodies, allowing researchers to visualize antigen-antibody interactions when paired with chromogenic or chemiluminescent substrates. Its high specificity minimizes cross-reactivity with other rat IgG subclasses (e.g., IgG1. IgG2b, IgG2c) or immunoglobulins from other species, ensuring reliable results.
Applications include quantifying immune responses, tracking protein expression, and validating disease biomarkers. Proper use requires optimization of dilution ratios and blocking steps to reduce background noise. Stored at 4°C for short-term or -20°C for long-term stability, it remains a critical tool in biomedical research for its sensitivity, versatility, and compatibility with high-throughput workflows.
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