| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Mouse anti Rat IgG1-HRP，Mouse anti Rat IgG1 HRP |
| Conjugation | HRP |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Secondary Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Rat IgG1 |
| Formulation | Purified antibody in PBS with 0.01% thimerosal. |
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以下是关于Rat IgG1-HRP抗体的示例参考文献(注：以下文献为示例性质，实际引用请通过学术数据库核实)：
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1. **文献名称**: *Antibody Labeling Techniques for Enhanced Immunoassay Sensitivity*
**作者**: Harlow, E., & Lane, D.
**摘要**: 该文献系统阐述了HRP标记抗体的制备与优化方法，其中提到Rat IgG1-HRP在Western Blot中的应用，强调其低交叉反应性及适用于高背景样本检测的特性。
2. **文献名称**: *Evaluation of Species-Specific Secondary Antibodies in Multiplex ELISA*
**作者**: Smith, J. et al.
**摘要**: 研究比较多种二抗性能，指出Rat IgG1-HRP在小鼠模型样本中表现出优异特异性，可减少非目标蛋白干扰，适用于多重ELISA检测体系。
3. **文献名称**: *Optimizing Immunohistochemical Staining in Neural Tissues*
**作者**: Lee, C. et al.
**摘要**: 探讨Rat IgG1-HRP在大脑切片染色中的参数优化，包括封闭剂浓度和显色时间，证明其在神经元标记中的高分辨率与低背景优势。
4. **文献名称**: *HRP-Conjugated Antibodies in Diagnostic Applications: A Meta-Analysis*
**作者**: Johnson, M. & Patel, R.
**摘要**: 综述HRP标记抗体的临床应用，提及Rat IgG1-HRP在自动化诊断平台中的稳定性和批次一致性，支持其作为标准化试剂的潜力。
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**建议**：实际研究中，可通过PubMed、Google Scholar等平台以关键词“Rat IgG1-HRP antibody”、“HRP-conjugated secondary antibody”检索最新文献，重点关注应用方法学或性能验证类文章。
Rat IgG1-HRP antibody is a type of secondary antibody conjugated to horseradish peroxidase (HRP), widely used in immunoassays for detecting rat-derived primary antibodies. Rat IgG1 is a subclass of immunoglobulin G (IgG) commonly produced in rats during immune responses. As a secondary antibody, it specifically binds to the Fc region of rat IgG1 primary antibodies, enabling signal amplification in techniques like Western blotting, ELISA, and immunohistochemistry. The HRP enzyme catalyzes colorimetric, chemiluminescent, or fluorescent reactions when exposed to substrates, allowing visualization of target proteins or antigens.
These antibodies are typically raised in hosts like goats or rabbits immunized with rat IgG1. followed by purification and HRP conjugation. Their specificity is validated to minimize cross-reactivity with immunoglobulins from other species or IgG subclasses. Rat IgG1-HRP antibodies are essential in dual-labeling experiments or when working with rat monoclonal primary antibodies, particularly in immunology and cancer research. Quality control ensures batch-to-batch consistency, high sensitivity, and low background noise. Proper storage at -20°C and avoidance of repeated freeze-thaw cycles are critical for maintaining activity. Their versatility and reliability make them indispensable tools in biomedical research and diagnostic applications.
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