| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Mouse anti Rat IgG2b-HRP，Mouse anti Rat IgG2b HRP |
| Conjugation | HRP |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Secondary Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Rat IgG2b |
| Formulation | Purified antibody in PBS with 0.01% thimerosal. |
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以下是3条关于Rat IgG2b-HRP抗体的示例参考文献(内容为模拟生成，非真实文献)：
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1. **文献名称**：*Optimization of Rat IgG2b-HRP Conjugates for Enhanced Sensitivity in Western Blotting*
**作者**：Smith J.A., et al.
**摘要**：本研究评估了不同标记比例的Rat IgG2b-HRP抗体在Western blot中的检测性能，发现通过优化HRP与抗体的偶联比例，可显著提高低丰度靶蛋白的检测灵敏度，同时降低非特异性背景信号。
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2. **文献名称**：*Cross-reactivity Analysis of Commercial Rat IgG2b-HRP Antibodies in Murine Tissue Models*
**作者**：Chen L., & Müller R.
**摘要**：通过比较5种市售Rat IgG2b-HRP抗体在小鼠组织切片中的交叉反应性，揭示了不同品牌抗体在免疫组化(IHC)中的特异性差异，并提出选择高特异性抗体的关键参数。
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3. **文献名称**：*A Novel ELISA Protocol Using Rat IgG2b-HRP for Quantifying Inflammatory Cytokines*
**作者**：Kumar S., et al.
**摘要**：开发了一种基于Rat IgG2b-HRP二抗的改良ELISA方案，用于精确检测血清中IL-6和TNF-α水平，验证了其在低至1 pg/mL浓度下的线性响应和稳定性。
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(注：如需真实文献，建议通过PubMed或Google Scholar检索关键词如"Rat IgG2b-HRP antibody application"或联系抗体供应商获取技术手册引用。)
Rat IgG2b-HRP (horseradish peroxidase) antibody is a secondary antibody widely used in immunological assays to detect rat-derived primary antibodies of the IgG2b subclass. Produced in host species like goats or rabbits, it specifically binds to the Fc region of rat IgG2b, enabling targeted detection in techniques such as ELISA, western blotting, and immunohistochemistry. The conjugation of HRP allows colorimetric, chemiluminescent, or fluorescent signal generation upon substrate addition, facilitating visualization of antigen-antibody interactions.
This antibody is valued for its high specificity, minimizing cross-reactivity with other rat IgG subclasses (e.g., IgG1. IgG2a) or immunoglobulins from unrelated species when properly validated. Its applications span biomedical research, including autoimmune studies, infectious disease modeling, and protein expression analysis. Quality control typically includes affinity purification and adsorption steps to reduce non-specific binding. Optimal performance requires titration to balance sensitivity and background noise. As rat IgG2b is less abundant than IgG1 or IgG2a in immune responses, this antibody is particularly useful in models where IgG2b-dominated responses are expected, such as certain vaccine studies or hybridoma-derived monoclonal antibody detection. Proper storage at -20°C with avoidance of freeze-thaw cycles preserves enzymatic activity.
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