| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Mouse anti Rat IgG2c-HRP，Mouse anti Rat IgG2c HRP |
| Conjugation | HRP |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Secondary Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Rat IgG2c |
| Formulation | Purified antibody in PBS with 0.01% thimerosal. |
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以下是3篇关于Rat IgG2c-HRP抗体的模拟参考文献示例(注：文献为虚构示例，实际引用需查询真实数据库)：
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1. **文献名称**: *"Development and Validation of Rat IgG2c-HRP for Enhanced Sensitivity in Autoantibody Detection"*
**作者**: Müller, A., et al.
**摘要**: 本研究报道了一种高灵敏度的Rat IgG2c-HRP抗体的制备及优化方法，通过改良偶联工艺提升其在Western blot中的信号强度。实验证明，该抗体在系统性红斑狼疮(SLE)小鼠模型中能特异性识别血清IgG2c亚型，降低非特异性背景。
2. **文献名称**: *"Comparative Analysis of Secondary Antibodies in Multiplex Immunoassays: Focus on Rat IgG2c-HRP"*
**作者**: Chen, L., & Watanabe, K.
**摘要**: 文章比较了不同HRP标记二抗(包括IgG1、IgG2a、IgG2c)在流式细胞术和ELISA中的性能，发现Rat IgG2c-HRP在检测低丰度抗原时具有更高的信噪比，尤其在Th1/Th2细胞因子谱分析中表现优异。
3. **文献名称**: *"Application of Rat IgG2c-HRP in Neuroinflammatory Disease Models"*
**作者**: Gupta, R., et al.
**摘要**: 在实验性自身免疫性脑脊髓炎(EAE)模型中，作者利用Rat IgG2c-HRP抗体定位中枢神经系统内的IgG沉积，验证了其与HRP底物TMB的兼容性，为神经炎症的病理机制研究提供了可靠工具。
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建议通过PubMed、Google Scholar等平台，以关键词“Rat IgG2c-HRP antibody”或“IgG2c isotype detection”检索真实文献。实际研究中需结合具体实验场景(如宿主物种、检测方法)筛选抗体参考文献。
**Background of Rat IgG2c-HRP Antibody**
The Rat IgG2c-HRP antibody is a horseradish peroxidase (HRP)-conjugated secondary antibody specifically designed to detect rat immunoglobulin G2c (IgG2c) primary antibodies in immunoassays. Rat IgG2c, part of the IgG subclass family, shares structural similarities with mouse IgG2a/c and plays roles in immune responses, including complement activation and Fc receptor binding.
This antibody is typically produced in hosts like goats or rabbits by immunizing them with rat IgG2c fragments. Post-purification, it is conjugated to HRP, an enzyme that catalyzes substrate reactions (e.g., TMB or chemiluminescent agents) to generate detectable signals in techniques like ELISA, Western blotting, or immunohistochemistry.
The IgG2c subclass in rats is associated with Th1-biased immune responses, distinguishing it from other IgG subclasses (e.g., IgG1. IgG2a, IgG2b). The Rat IgG2c-HRP antibody’s specificity ensures minimal cross-reactivity with other rat IgG subtypes or immunoglobulins from unrelated species, making it ideal for experiments requiring precise detection.
Applications include autoimmune disease studies, infectious disease research, and monoclonal antibody characterization. Proper controls are recommended to validate specificity, and storage typically follows -20°C protocols to preserve enzymatic activity. Its utility lies in combining high sensitivity with the versatility of HRP-based detection systems.
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