| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SAP18 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SAP18抗体的3篇文献示例(注：以下内容为示例性概括，非真实文献)：
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1. **文献名称**: *SAP18 Mediates Transcriptional Repression through Interaction with the Sin3/HDAC Complex*
**作者**: Zhang, Y. et al.
**摘要**: 研究证实SAP18通过与Sin3/HDAC复合体结合参与转录抑制，利用SAP18抗体进行免疫共沉淀实验，揭示其在组蛋白去乙酰化及基因沉默中的作用机制。
2. **文献名称**: *SAP18 Associates with mRNA Splicing Factors and Regulates Alternative Splicing*
**作者**: Cheng, C. et al.
**摘要**: 通过SAP18抗体的免疫荧光染色和RNA pull-down实验，发现SAP18与hnRNP蛋白互作，调控特定前体mRNA的剪接过程，影响细胞应激反应。
3. **文献名称**: *SAP18 Promotes Apoptosis via Caspase-Dependent Pathways in Cancer Cells*
**作者**: Wang, L. et al.
**摘要**: 使用SAP18抗体的Western blot分析显示，SAP18在癌细胞中高表达，并通过激活caspase通路诱导凋亡，提示其作为潜在肿瘤治疗靶点。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“SAP18 antibody”、“SAP18 function”检索近期研究。
The SAP18 (Sin3A-associated protein 18) antibody is a research tool used to study the SAP18 protein, a component of the SIN3 transcriptional regulatory complex. SAP18. with a molecular weight of approximately 18 kDa, plays a role in gene silencing by recruiting histone deacetylases (HDACs) to chromatin, facilitating chromatin condensation and transcriptional repression. It interacts with SIN3. HDACs, and other co-repressors, linking epigenetic modifiers to sequence-specific DNA-binding proteins.
SAP18 is implicated in diverse cellular processes, including apoptosis, RNA splicing, and stress responses. Studies highlight its involvement in developmental regulation, cancer (via tumor suppressor pathways), and neurological disorders. Dysregulation of SAP18-associated complexes may contribute to aberrant gene expression in diseases.
The SAP18 antibody is widely used in techniques like Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interactions. Researchers utilize it to explore SAP18's role in epigenetic regulation, particularly in contexts like DNA damage response or differentiation. Commercial SAP18 antibodies are typically validated for specificity, often targeting conserved regions across species. However, batch variability and cross-reactivity with homologous proteins require careful experimental controls. Its applications span molecular biology, cancer research, and neurobiology, offering insights into chromatin remodeling mechanisms.
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