| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NADK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NADK抗体的参考文献示例(文献信息为示例性内容，非真实文献)：
1. **"NADK-mediated NADPH production regulates glioblastoma cell proliferation"**
- 作者：Li, X. et al. (2015)
- 摘要：研究使用特异性NADK抗体检测胶质母细胞瘤中NADK蛋白表达，发现其通过促进NADPH合成驱动肿瘤细胞增殖，提示NADK可能成为治疗靶点。
2. **"Antibody-based profiling of NADK isoforms in metabolic disorders"**
- 作者：Hoxhaj, G. et al. (2018)
- 摘要：开发高特异性NADK抗体，揭示不同组织及代谢疾病模型中NADK异构体表达的差异，证实NADK在脂质代谢中的调控作用。
3. **"NADK2 deficiency identified by immunofluorescence staining in mitochondrial dysfunction"**
- 作者：Lee, S. et al. (2020)
- 摘要：利用NADK2抗体进行免疫荧光分析，发现其在线粒体中的定位异常与氧化应激和细胞凋亡相关，为遗传性代谢疾病提供分子机制证据。
4. **"NADK1 antibody validation in breast cancer progression studies"**
- 作者：Chen, Y. et al. (2019)
- 摘要：通过免疫组化和Western blot验证商业化NADK1抗体的可靠性，发现其在乳腺癌组织中高表达并与患者预后不良相关。
(注：以上文献为示例，实际引用时请核实真实文献信息。)
The NADK antibody is a crucial tool for studying nicotinamide adenine dinucleotide kinase (NADK), an enzyme responsible for catalyzing the phosphorylation of NAD⁺ to generate NADP⁺, a vital cofactor in cellular redox reactions and biosynthetic pathways. NADK plays a central role in maintaining the NADP⁺/NADPH pool, essential for lipid synthesis, antioxidant defense, and cellular stress responses. Dysregulation of NADK has been implicated in metabolic disorders, cancer, and neurodegenerative diseases, driving interest in its functional characterization.
NADK antibodies are specifically designed to detect and quantify NADK protein levels in various experimental models, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies enable researchers to investigate NADK expression patterns across tissues, its subcellular localization, and its modulation under physiological or pathological conditions, such as oxidative stress or metabolic reprogramming in tumors.
Commercially available NADK antibodies are typically produced in hosts like rabbits or mice, targeting specific epitopes within human, mouse, or rat NADK isoforms. Validation includes testing for specificity, sensitivity, and cross-reactivity to ensure reliability. Their applications extend to drug discovery, biomarker studies, and mechanistic research into NADK-related pathways, offering insights into therapeutic strategies targeting NADPH-dependent processes. Overall, NADK antibodies are indispensable for elucidating the enzyme's role in health and disease.
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