关键信息
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基因名
PRC1
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简介
PRC1 is a key regulator of cytokinesis, coordinating midzone formation and successful cell division by cross-linking microtubules and recruiting essential proteins such as KIF14, PLK1 and ECT2. Its effects extend to tumorigenesis, promoting bladder cancer cell proliferation and inhibiting apoptosis. PRC1 Protein, Human (sf9, His) is the recombinant human-derived PRC1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- 应用
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别名
Protein regulator of cytokinesis 1; PRC1
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种属
Human
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表达系统
Baculovirus
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43663
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表达区间
M1-S620
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蛋白长度
Full Length
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分子量
75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PRC1 (Polycomb Repressive Complex 1) is a crucial protein complex that plays a significant role in the regulation of gene expression, cellular differentiation, and development. It mediates transcriptional repression primarily through histone modification, specifically by catalyzing monoubiquitination of histone H2A at lysine 119. This modification leads to the compaction of chromatin, thereby silencing target genes. The study of PRC1 has gained attention in recent years due to its involvement in various biological processes, including stem cell maintenance, embryonic development, and the progression of certain cancers. Dysregulation of PRC1 components has been linked to tumorigenesis, making it a potential target for therapeutic interventions. Furthermore, understanding its complex interactions with other proteins and epigenetic regulators is critical for unraveling its role in cellular identity and lineage specification. Recent advances in structural biology and biochemistry have facilitated the exploration of PRC1's architecture and functionality, paving the way for novel insights into how the epigenome is dynamically regulated during development and pathological conditions. Ongoing research aims to elucidate the mechanistic details of PRC1 action and its broader implications in health and disease, thus highlighting the importance of this complex in the field of epigenetics and molecular biology.
