关键信息
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基因名
CDK1-CCNB1
- 应用
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别名
CDC2; CDC28A; CDK1; CDC28-A; CDKN1; P34CDC2; p34 protein kinase; Cell Division Control Protein 2 Homolog; Cell Division Cycle 2,G1 To S And G2 To M
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P06493
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表达区间
Arg59~Ser182
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分子量
15kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CDK1 (Cyclin-dependent kinase 1) is a crucial regulator of the cell cycle, particularly in the transition from the G2 phase to mitosis. It functions in association with its regulatory partner, Cyclin B1 (CCNB1), forming a complex that is essential for the initiation of mitotic events. The CDK1-CCNB1 complex phosphorylates various substrates, resulting in chromatin condensation, spindle assembly, and ultimately, cell division. Dysregulation of CDK1 activity has been implicated in various cancers, where aberrant cell division contributes to tumorigenesis. Research into the CDK1-CCNB1 complex is important not only for understanding fundamental cell cycle mechanisms but also for identifying potential therapeutic targets in cancer treatment. Recombinant proteins of CDK1 and CCNB1 facilitate the study of their biochemical properties and interactions, allowing researchers to investigate how alterations in this complex might affect mitotic progression and contribute to disease. By analyzing the structure-function relationships of CDK1 and CCNB1, researchers aim to uncover new insights into cell cycle regulation and its implications in cancer biology. Additionally, the development of small molecules targeting the CDK1-CCNB1 complex may lead to novel therapeutic strategies for cancer patients, making the study of this recombinant protein complex highly relevant in both basic and applied biomedical research.
