关键信息
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基因名
RGS5
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简介
RGS5, an adept signal transduction modulator, enhances G protein alpha subunit GTPase activity, promoting their transition to the inactive GDP-bound state. Interacting with G(i)-alpha and G(o)-alpha, it selectively excludes G(s)-alpha, highlighting its precise role in regulating specific G protein subunits and fine-tuning cellular signaling pathways. RGS5 Protein, Human (His) is the recombinant human-derived RGS5 protein, expressed by E. coli , with N-6*His labeled tag.
- 应用
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别名
Regulator of G-protein signaling 5; RGS5
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15539
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表达区间
M1-K181
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氨基酸序列
MCKGLAALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYNEKPEKPAKTQKTSLDEALQWRDSLDKLLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFYQELIK
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蛋白长度
Full Length
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分子量
25 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RGS5 (Regulator of G-protein Signaling 5) is a member of the RGS protein family, which plays a crucial role in regulating G-protein-coupled receptor (GPCR) signaling pathways. Research into RGS5 has gained momentum due to its involvement in several physiological processes, including vascular development, smooth muscle contractility, and angiogenesis. Abnormal expression of RGS5 has been implicated in various pathological conditions, such as hypertension, heart failure, and certain types of cancer, making it a potential therapeutic target. The recombinant expression of RGS5 protein allows for detailed biochemical characterization and functional studies, aiding in the understanding of its role in cell signaling. Additionally, this research has implications for drug development, as modulating RGS5 activity could offer new strategies for treating diseases linked to GPCR dysregulation. By studying the structure and function of RGS5 through recombinant protein techniques, researchers aim to unveil the molecular mechanisms by which RGS5 exerts its effects on cellular signaling, ultimately contributing to advancements in medical therapies and interventions.
