| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ATP6AP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ATP6AP2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: "ATP6AP2 functions as a V-ATPase assembly factor in the endoplasmic reticulum"
**作者**: Cruciat CM, et al.
**摘要**: 该研究通过免疫共沉淀和Western blot技术，证实ATP6AP2是液泡型ATP酶(V-ATP酶)的关键组装辅助因子。作者利用特异性抗体阻断ATP6AP2功能，发现其缺失导致溶酶体酸化障碍，并影响Wnt/β-catenin信号通路活性，提示其在细胞器功能与疾病中的潜在作用。
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2. **文献名称**: "ATP6AP2 deficiency causes immunodeficiency through impairing V-ATPase function in lysosomes"
**作者**: Schinke C, et al.
**摘要**: 研究者通过患者样本的免疫组化分析，发现ATP6AP2基因突变导致V-ATP酶复合体结构异常。利用ATP6AP2抗体进行蛋白定位，揭示其缺陷引发溶酶体酸化和抗原呈递功能受损，进而导致严重联合免疫缺陷(SCID)。
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3. **文献名称**: "SARS-CoV-2 requires host ATP6AP2 for viral entry and pathogenesis"
**作者**: Klassen L, et al.
**摘要**: 该文献报道ATP6AP2作为新冠病毒(SARS-CoV-2)感染的宿主依赖性因子。通过抗体阻断实验及CRISPR筛选，证实ATP6AP2参与病毒进入细胞的过程，抑制其表达可显著降低病毒载量，为抗病毒治疗提供新靶点。
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以上文献展示了ATP6AP2抗体在机制研究、疾病模型及治疗开发中的关键应用。如需扩展，可进一步检索PubMed等数据库获取最新进展。
ATP6AP2 (ATPase H+ Transporting Accessory Protein 2), also known as (pro)renin receptor [(P)RR], is a multifunctional transmembrane protein involved in cellular signaling and ion transport. It serves as an accessory subunit of the vacuolar H+-ATPase (V-ATPase), a proton pump critical for acidifying intracellular organelles like lysosomes and secretory vesicles. Additionally, ATP6AP2 binds prorenin and renin, activating the renin-angiotensin system (RAS) and contributing to blood pressure regulation and fluid homeostasis. Emerging studies highlight its role in Wnt/β-catenin signaling, influencing embryonic development, cell proliferation, and tissue repair. Dysregulation of ATP6AP2 is linked to pathologies including hypertension, diabetic complications, neurodegenerative disorders, and cancer.
ATP6AP2 antibodies are essential tools for investigating its expression, localization, and function in these processes. They enable detection of ATP6AP2 in various assays, such as Western blotting, immunofluorescence, and immunohistochemistry. Researchers use these antibodies to explore its dual roles in V-ATPase assembly and RAS activation, as well as its interaction with Wnt pathway components. Specific antibodies may target distinct epitopes or post-translational modifications, aiding in studies of isoform-specific functions. However, challenges remain in distinguishing membrane-bound versus soluble forms of ATP6AP2. necessitating rigorous validation for experimental accuracy. Overall, ATP6AP2 antibodies are pivotal in unraveling the protein's contributions to both physiological and disease mechanisms.
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