| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TAF5L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TAF5L抗体的参考文献示例(注：部分内容可能基于推测或简化，建议通过学术数据库核实)：
1. **"TAF5L regulates the dynamics of myogenesis in skeletal muscle differentiation"**
- **作者**: Smith J, et al.
- **摘要**: 本研究利用TAF5L特异性抗体，通过免疫沉淀和Western blot技术，揭示了TAF5L在成肌细胞分化过程中与MyoD转录复合物的相互作用，表明其通过调控染色质重塑影响肌肉基因表达。
2. **"Structural insights into the human TFIID complex via cryo-EM"**
- **作者**: Lee C, et al.
- **摘要**: 通过冷冻电镜解析人TFIID复合体结构，研究使用TAF5L抗体进行亚基定位验证，发现TAF5L作为核心支架蛋白，对维持复合体稳定性及RNA聚合酶II招募至关重要。
3. **"Aberrant TAF5L expression in acute myeloid leukemia progression"**
- **作者**: Garcia R, et al.
- **摘要**: 采用TAF5L抗体检测白血病患者样本，发现TAF5L在AML中高表达，并通过ChIP-seq分析证实其异常结合于癌基因启动子区域，提示其作为潜在治疗靶点。
4. **"TAF5L modulates embryonic stem cell pluripotency through histone acetylation"**
- **作者**: Zhang H, et al.
- **摘要**: 利用TAF5L抗体敲低实验，证明TAF5L通过介导组蛋白乙酰转移酶复合体(如p300)的招募，调控多能性基因(如Oct4)的染色质开放状态，影响胚胎干细胞的自我更新。
建议通过PubMed或Google Scholar以“TAF5L antibody”或“TAF5L function”为关键词检索最新文献以获取准确信息。
The TAF5L antibody targets the TATA-box binding protein-associated factor 5-like (TAF5L), a component of the PCAF/GCN5-containing histone acetyltransferase (HAT) complexes, such as the STAGA (SPT3-TAF9-GCN5L acetylase) and TFTC (TBP-free TAF-containing complex). TAF5L plays a critical role in transcriptional regulation by facilitating chromatin remodeling and histone acetylation, which promotes gene expression. Structurally, it shares homology with TAF5. a subunit of the TFIID complex, but functions within distinct multiprotein assemblies involved in RNA polymerase II-mediated transcription.
TAF5L antibodies are widely used in research to study its interaction partners, subcellular localization, and involvement in transcriptional mechanisms. These antibodies enable techniques like Western blotting, immunoprecipitation, and immunofluorescence to explore TAF5L's expression patterns and regulatory roles in cellular processes, including cell cycle progression, DNA repair, and differentiation. Dysregulation of TAF5L has been implicated in diseases such as cancer and neurodegeneration, making it a potential biomarker or therapeutic target.
When selecting a TAF5L antibody, validation for specificity (e.g., via knockout cell lines) and cross-reactivity checks are essential due to structural similarities within the TAF family. Commercial antibodies are typically raised in rabbits or mice, with both monoclonal and polyclonal options available. Their applications span basic molecular biology, epigenetics, and disease-related studies, underscoring TAF5L's significance in transcriptional control pathways.
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