| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human VMP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于VMP1抗体的3篇模拟参考文献示例(基于公开研究领域知识，具体文献需通过学术数据库验证)：
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1. **文献名称**：*VMP1 regulates autophagy induction and lysosomal function in pancreatic cancer cells*
**作者**：Molejon, M.I., et al.
**摘要**：本研究利用VMP1特异性抗体，揭示了VMP1在胰腺癌细胞自噬激活中的关键作用，证明其通过调控自噬体与溶酶体融合影响肿瘤细胞存活，为靶向自噬的癌症治疗提供依据。
2. **文献名称**：*VMP1 as a novel biomarker for early pancreatic ductal adenocarcinoma detection*
**作者**：Ropolo, A., et al.
**摘要**：通过免疫组化(VMP1抗体)和qPCR分析，发现VMP1在胰腺导管腺癌(PDAC)早期组织中显著高表达，提示其可作为早期诊断的生物标志物，并与患者预后不良相关。
3. **文献名称**：*VMP1 antibody-based imaging reveals dynamic subcellular localization during autophagosome formation*
**作者**：Grasso, D., et al.
**摘要**：利用高特异性VMP1抗体进行活细胞成像，首次可视化VMP1在自噬体形成过程中的动态定位，证实其与ER膜及自噬相关蛋白(如LC3)的相互作用机制。
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**说明**：以上文献为基于VMP1研究领域的模拟示例，实际引用需通过PubMed、Web of Science等平台检索真实文献(关键词：VMP1 antibody, autophagy, pancreatic cancer)。如需具体文章，可提供发表年份或研究主题进一步筛选。
The Vacuole Membrane Protein 1 (VMP1) antibody is a research tool targeting the VMP1 protein, a transmembrane protein implicated in critical cellular processes such as autophagy, membrane trafficking, and organelle biogenesis. Initially identified for its role in pancreatic acinar cell vacuole formation, VMP1 is now recognized as a key regulator of autophagosome formation, facilitating interactions between the endoplasmic reticulum (ER) and isolation membranes during autophagy. Its expression is essential for maintaining cellular homeostasis under stress conditions, including nutrient deprivation or endoplasmic reticulum stress.
VMP1 antibodies are widely used in molecular and cell biology to study autophagy dynamics, ER-membrane contact sites, and secretory pathways. They enable detection and localization of VMP1 via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of VMP1 has been linked to diseases such as cancer, neurodegeneration, and pancreatitis, making these antibodies valuable for investigating disease mechanisms. For example, reduced VMP1 levels correlate with impaired autophagy in cancer cells, potentially influencing tumor progression and therapy resistance.
Commercial VMP1 antibodies are typically raised in rabbits or mice, with validation in knockout models to ensure specificity. Their applications extend to preclinical models, including studies on zebrafish and mice, to explore developmental and pathological roles of VMP1. Ongoing research continues to uncover its interactions with autophagy-related proteins (e.g., ATG14. Beclin-1) and its broader impact on cellular stress responses.
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