| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NEB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NEB抗体的3篇示例参考文献(注：以下为模拟示例，实际文献需根据具体研究检索)：
1. **文献名称**：*Application of NEB monoclonal antibodies in Western blot analysis of histone modifications*
**作者**：Smith J, et al.
**摘要**：本研究使用NEB公司开发的H3K4me3特异性单克隆抗体，成功在小鼠脑组织样本中检测到组蛋白修饰信号。该抗体表现出高特异性和低背景噪声，验证了其在表观遗传学研究中的可靠性。
2. **文献名称**：*Comparative evaluation of NEB anti-Cas9 antibodies in CRISPR genome editing validation*
**作者**：Chen L, et al.
**摘要**：通过对比多种商业抗体，发现NEB提供的Cas9抗体在免疫沉淀(IP)实验中具有最佳靶标结合效率，为CRISPR编辑细胞的蛋白水平验证提供了优化方案。
3. **文献名称**：*Development of a diagnostic panel using NEB p53 antibodies for early cancer screening*
**作者**：Gupta R, et al.
**摘要**：利用NEB生产的人源p53磷酸化抗体组合，建立了基于免疫组化的肿瘤标志物检测体系，在结直肠癌活检样本中实现了90%以上的检测一致性。
(提示：实际研究中建议通过PubMed或NEB官网"CiteAb"工具获取具体产品相关文献)
Neighborhood Antibodies (NEB) are specialized tools in molecular biology, primarily developed to target and detect specific protein epitopes with high precision. Originating from advancements in recombinant DNA technology and hybridoma techniques, NEB antibodies are widely utilized in research, diagnostics, and therapeutic development. Their design often leverages phage display or transgenic animal platforms, enabling the generation of highly specific monoclonal or polyclonal antibodies against challenging antigens, including post-translationally modified proteins or low-abundance targets.
NEB antibodies are rigorously validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry, ensuring reproducibility across experimental conditions. A key feature is their compatibility with knockout/knockdown validation, confirming target specificity. They are also engineered for reduced cross-reactivity, enhancing signal-to-noise ratios in complex biological samples.
Commercial providers, including New England Biolabs (NEB), emphasize quality control through ISO certification and batch-to-batch consistency, making them reliable for high-stakes research. NEB’s product line often includes antibodies conjugated to enzymes or fluorophores, streamlining workflow integration. With the rise of personalized medicine, NEB antibodies increasingly support biomarker discovery and therapeutic targeting, underscoring their evolving role in both basic science and translational applications.
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