| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human STX8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于STX8抗体的3篇参考文献示例(注：内容为模拟虚构，仅作格式参考)：
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1. **文献名称**：*"Characterization of Syntaxin-8 in Endosomal Trafficking Using a Novel Polyclonal Antibody"*
**作者**：Zhang L, et al. (2015)
**摘要**：本研究开发并验证了一种兔源多克隆STX8抗体，通过免疫印迹和免疫荧光证实其在多种哺乳动物细胞系中的特异性。结果显示，STX8在内体膜融合过程中与VAMP7形成功能复合物，为溶酶体运输机制提供了新见解。
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2. **文献名称**：*"STX8 Antibody-Based Detection of Aberrant Synaptic Vesicle Recycling in Neurodegenerative Models"*
**作者**：Gupta S, et al. (2018)
**摘要**：利用小鼠单克隆STX8抗体(克隆号：3A2)，作者揭示了STX8在阿尔茨海默病模型中与突触囊泡循环异常的关联。抗体经敲除细胞系验证后，成功用于脑组织切片染色及蛋白质互作分析。
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3. **文献名称**：*"Comparative Analysis of Commercial STX8 Antibodies for Colorectal Cancer Biomarker Studies"*
**作者**：Wang Y, et al. (2020)
**摘要**：系统性评估了四种市售STX8抗体(来自Abcam、CST、Sigma和Santa Cruz)在结直肠癌组织中的性能，发现不同克隆的敏感性和特异性差异显著，推荐Abcam货号#12345用于IHC定量研究。
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(注：以上文献为模拟生成，实际引用需查询真实数据库如PubMed。)
The STX8 antibody is a research tool designed to detect and study Syntaxin-8 (STX8), a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment Protein Receptor) protein family. STX8 plays a critical role in intracellular membrane trafficking, particularly in endosomal and lysosomal fusion events. It functions as a target-SNARE (t-SNARE), interacting with vesicle-SNAREs (v-SNAREs) to mediate the docking and fusion of transport vesicles with target membranes. Structurally, STX8 contains a conserved SNARE domain and a C-terminal transmembrane anchor, enabling its integration into lipid bilayers.
Research on STX8 has highlighted its involvement in endosome maturation, autophagy, and receptor sorting, with implications in neurodegenerative diseases, cancer, and immune regulation. For example, STX8 interacts with proteins like VAMP7 and SNAP23 to regulate lysosomal function and secretory pathways. Antibodies against STX8 are typically developed in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of the protein. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to explore STX8 expression, localization, and molecular interactions.
The availability of STX8 antibodies has advanced studies on membrane trafficking mechanisms, offering insights into cellular homeostasis and disease pathogenesis. However, specificity validation (e.g., knockout controls) remains essential due to potential cross-reactivity with other SNARE family members.
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