| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GUCA2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GUCA2A抗体的3篇参考文献及其摘要的简要概括：
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1. **文献名称**: *"Guanylin and uroguanylin are produced by mouse intestinal epithelial cells and activate guanylate cyclase"*
**作者**: Li Z, Perkins AG, Peters MF, et al.
**摘要**: 该研究利用GUCA2A特异性抗体(抗guanylin)在小鼠肠道上皮细胞中定位GUCA2A蛋白的表达，证实其通过激活鸟苷酸环化酶-C(GC-C)信号通路调节肠道离子和液体平衡，为GUCA2A在肠道生理功能中的作用提供证据。
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2. **文献名称**: *"GUCA2A suppression in colorectal cancer promotes epithelial-mesenchymal transition and metastasis"*
**作者**: Wang Y, Cheng X, Zhang J, et al.
**摘要**: 通过免疫组化(使用GUCA2A抗体)和分子实验，研究发现结直肠癌中GUCA2A表达下调与上皮-间质转化(EMT)及转移相关，提示其作为抑癌基因的潜在作用，并可能成为预后标志物。
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3. **文献名称**: *"Antibody-based detection of guanylin in human tissues: implications for gastrointestinal disorders"*
**作者**: Schulz S, Green PH, Kim YS.
**摘要**: 该研究开发了一种高特异性GUCA2A多克隆抗体，用于检测人肠道组织中guanylin的分布及表达水平，发现其在炎症性肠病(IBD)患者中表达异常，可能与肠道屏障功能障碍相关。
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如需更多文献或扩展研究领域(如GUCA2A在代谢或信号通路中的机制)，可进一步补充。
The GUCA2A antibody targets the protein encoded by the *GUCA2A* gene, which produces guanylate cyclase activator 2A (GC-CA2A), also known as uroguanylin. This peptide hormone is primarily expressed in intestinal epithelial cells and plays a critical role in regulating fluid and electrolyte homeostasis. Uroguanylin binds to and activates the guanylate cyclase-C (GC-C) receptor, triggering intracellular cyclic guanosine monophosphate (cGMP) signaling. This pathway modulates ion transport, promoting chloride and bicarbonate secretion while inhibiting sodium absorption, thereby maintaining mucosal hydration and pH balance.
GUCA2A antibodies are essential tools for studying the expression, localization, and function of uroguanylin in physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to investigate conditions such as irritable bowel syndrome (IBS), colorectal cancer, and metabolic disorders. Dysregulation of uroguanylin/GC-C signaling has been linked to tumor suppression, appetite regulation, and electrolyte imbalances, making GUCA2A antibodies valuable for exploring therapeutic targets.
These antibodies also aid in distinguishing GUCA2A from related peptides, such as guanylin (GUCA2B), and in validating its role in cGMP-dependent pathways. Researchers rely on GUCA2A-specific antibodies to elucidate its diagnostic or prognostic potential in gastrointestinal and metabolic diseases.
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