| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human VRK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与VRK1抗体应用相关的参考文献及其摘要概述：
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1. **文献标题**: "VRK1 regulates cisplatin resistance via the NRF2 antioxidant pathway in neuroblastoma"
**作者**: Valencia-Sama, I. et al.
**摘要**: 本研究利用VRK1抗体进行Western blot和免疫荧光实验，发现VRK1通过调控NRF2通路介导神经母细胞瘤对顺铂的耐药性。抗体检测显示，VRK1高表达与患者不良预后相关，提示其作为治疗靶点的潜力。
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2. **文献标题**: "VRK1 facilitates DNA damage response by phosphorylating and activating ATM kinase"
**作者**: Santos, C.R. et al.
**摘要**: 通过免疫共沉淀(使用VRK1抗体)和激酶活性实验，作者揭示VRK1在DNA损伤后磷酸化并激活ATM激酶，促进细胞周期检查点调控。该研究为VRK1在基因组稳定性中的作用提供了机制证据。
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3. **文献标题**: "Prognostic significance of VRK1 expression in colorectal cancer: an antibody-based immunohistochemical analysis"
**作者**: Campo-Caballero, D. et al.
**摘要**: 采用VRK1抗体对结直肠癌组织进行免疫组化染色，发现VRK1蛋白高表达与肿瘤侵袭性及患者生存期缩短显著相关，提示其可作为潜在的预后生物标志物。
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这些文献均通过VRK1抗体开展蛋白质功能研究，涵盖肿瘤耐药机制、DNA损伤应答及临床预后分析等领域。如需扩展，可进一步检索近年文献数据库(如PubMed)获取最新进展。
The VRK1 (Vaccinia-Related Kinase 1) antibody is a research tool designed to detect and study the VRK1 protein, a serine/threonine kinase critical in cellular processes such as cell cycle regulation, DNA damage response, and neuronal development. VRK1. part of the VRK kinase family, phosphorylates targets like histone H3 and p53. influencing chromatin remodeling, apoptosis, and genome stability. Dysregulation of VRK1 is linked to cancers (e.g., breast, lung) and neurological disorders (e.g., spinal muscular atrophy, microcephaly).
VRK1 antibodies are typically produced in hosts like rabbits or mice using immunogens such as recombinant VRK1 protein or synthetic peptides. They enable techniques including Western blotting, immunohistochemistry, and immunofluorescence to analyze VRK1 expression, localization, and interactions in tissues or cultured cells. Monoclonal antibodies offer high specificity, while polyclonal versions detect multiple epitopes, enhancing sensitivity.
Quality validation involves knockout cell controls, cross-reactivity tests, and comparison with published data. Beyond research, VRK1 antibodies hold therapeutic potential, aiding drug discovery for VRK1-associated diseases. Their development underscores VRK1's role as both a biomarker and a target in precision medicine.
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