| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PNP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PNP(嘌呤核苷磷酸化酶)抗体的参考文献示例，包括虚构的文献名称、作者及摘要概括：
1. **文献名称**：*Autoantibodies Against Purine Nucleoside Phosphorylase in Autoimmune Disorders*
**作者**：Smith J, et al.
**摘要**：本研究检测了自身免疫性疾病患者血清中抗PNP抗体的存在，发现其在系统性红斑狼疮(SLE)患者中的阳性率显著升高。抗体水平与疾病活动度相关，提示PNP可能成为SLE的新型生物标志物。
2. **文献名称**：*Development of a High-Sensitivity ELISA for PNP Detection Using Monoclonal Antibodies*
**作者**：Chen L, et al.
**摘要**：研究团队开发了一种基于单克隆抗体的高灵敏度ELISA方法，用于定量检测血清中的PNP酶。该方法在原发性免疫缺陷患者的诊断中表现出高特异性，为早期筛查提供了可靠工具。
3. **文献名称**：*PNP Deficiency and Antibody-Mediated Enzyme Inhibition: Implications for Immunodeficiency*
**作者**：Rodriguez M, et al.
**摘要**：文章探讨了PNP缺乏症患者中发现的抑制性抗体如何干扰酶活性，导致T细胞功能异常。通过体外实验证实，这些抗体可加剧代谢异常，为靶向治疗提供了理论依据。
4. **文献名称**：*Cross-Reactivity of Anti-PNP Antibodies with Viral Proteins in Chronic Infections*
**作者**：Kim S, et al.
**摘要**：研究发现，部分抗PNP抗体与某些病毒蛋白存在交叉反应，可能在慢性病毒感染(如EBV)中诱发自身免疫反应。这为理解感染后自身免疫并发症提供了新视角。
**注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
Purine nucleoside phosphorylase (PNP) antibodies are autoantibodies targeting the enzyme PNP, which plays a critical role in purine metabolism by catalyzing the phosphorolysis of nucleosides. While PNP deficiency is a rare autosomal recessive disorder causing severe immunodeficiency, PNP antibodies are primarily associated with autoimmune conditions rather than metabolic defects. These antibodies are notably linked to systemic sclerosis (SSc), particularly in patients with limited cutaneous involvement or overlap syndromes.
Clinically, PNP antibodies are detected in approximately 3–10% of SSc cases and are often co-expressed with other autoantibodies like anti-Th/To or anti-U3 RNP. Their presence is correlated with specific clinical features, including interstitial lung disease, pulmonary hypertension, and gastrointestinal manifestations. However, their pathogenetic role remains unclear; they may serve as biomarkers rather than direct contributors to tissue damage.
Detection methods include immunoprecipitation and line immunoassays. Despite their diagnostic utility, PNP antibodies are less characterized compared to other SSc-related autoantibodies (e.g., anti-topoisomerase I or anti-centromere). Ongoing research focuses on elucidating their epitope specificity and clinical prognostic value in autoimmune cohorts. Their identification aids in disease stratification and personalized management, though further studies are needed to define their mechanistic implications.
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