| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Alpha 2 macroglobulin purified from human plasma |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于A2M(α2-巨球蛋白)抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *"Alpha-2-Macroglobulin as a Novel Biomarker for Alzheimer's Disease"*
**作者**: Smith J, et al.
**摘要**: 该研究探讨了A2M在阿尔茨海默病中的作用，发现其抗体可特异性结合异常Aβ蛋白，抑制淀粉样斑块形成，并通过小鼠模型验证了A2M抗体对认知功能的保护作用。
2. **文献名称**: *"Targeting A2M in Cancer: Therapeutic Potential of Monoclonal Antibodies"*
**作者**: Lee H, et al.
**摘要**: 研究者开发了一种针对A2M的单克隆抗体，体外实验显示其能阻断A2M介导的肿瘤细胞侵袭和转移，并通过抑制TGF-β信号通路增强化疗敏感性。
3. **文献名称**: *"A2M Antibody-Based Detection of Chronic Inflammation in Autoimmune Disorders"*
**作者**: Patel R, et al.
**摘要**: 该研究利用A2M抗体建立了一种新型ELISA检测方法，发现血清A2M水平与类风湿性关节炎患者的炎症程度正相关，提示其作为炎症标志物的潜力。
4. **文献名称**: *"Structural Insights into A2M-Antibody Interactions for Therapeutic Design"*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜解析A2M与其抗体的复合物结构，揭示了抗体结合A2M关键功能域(如蛋白酶捕获区域)的分子机制，为开发靶向A2M的抑制剂奠定基础。
(注：以上文献为示例，实际研究中需根据具体需求检索真实发表的论文。)
**Background of A2M Antibodies**
Alpha-2-macroglobulin (A2M) is a large plasma glycoprotein and a key member of the protease inhibitor family, primarily involved in regulating proteolytic enzymes and innate immunity. It neutralizes a broad spectrum of proteases (e.g., matrix metalloproteinases, thrombin) by entrapping them through a unique "bait-and-trap" mechanism, forming complexes that are rapidly cleared via receptor-mediated endocytosis. A2M also modulates cytokine signaling, immune responses, and cellular repair processes.
A2M antibodies are tools used to detect or inhibit A2M in research and diagnostics. In autoimmune diseases (e.g., rheumatoid arthritis), elevated A2M-protease complexes correlate with inflammation, making these antibodies valuable for studying disease mechanisms. In neurodegenerative disorders like Alzheimer’s disease, A2M interacts with amyloid-β, implicating it in pathogenesis. Antibodies targeting A2M help explore its role in protein aggregation and clearance.
Therapeutic applications are emerging, particularly in osteoarthritis, where A2M’s anti-protease properties are harnessed to protect joint cartilage. Challenges include A2M’s structural complexity and cross-reactivity risks. Nonetheless, A2M antibodies remain pivotal in dissecting its multifaceted roles in homeostasis and disease.
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