| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ACTN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ACTN2抗体的3篇参考文献示例(内容为模拟虚构，仅供格式参考)：
1. **文献名称**: *"ACTN2 mutations in familial dilated cardiomyopathy: functional characterization of α-actinin-2 autoantibodies"*
**作者**: Guo W, et al.
**摘要**: 该研究探讨了ACTN2基因突变与家族性扩张型心肌病(DCM)的关联，发现患者体内存在特异性抗ACTN2抗体，这些抗体可能通过干扰心肌细胞骨架稳定性导致收缩功能障碍。
2. **文献名称**: *"Immunolocalization of α-actinin-2 in skeletal muscle: insights from antibody-based assays"*
**作者**: Henderson DM, et al.
**摘要**: 研究利用ACTN2特异性抗体进行免疫荧光分析，揭示了α-actinin-2在骨骼肌Z盘中的分布模式，并发现其在肌营养不良症患者中表达水平显著降低。
3. **文献名称**: *"The role of ACTN2 in cardiac sarcomere organization: evidence from antibody-mediated functional inhibition"*
**作者**: Lange S, et al.
**摘要**: 通过抗体阻断实验，研究发现α-actinin-2对心肌细胞肌节的组装至关重要，其功能异常可能导致心律失常和心肌肥厚。
(注：以上文献为示例，实际引用需根据PubMed或学术数据库检索真实发表内容。)
ACTN2 antibodies are immunological tools targeting alpha-actinin-2 (ACTN2), a structural protein critical for muscle function. ACTN2 belongs to the alpha-actinin family of actin-binding proteins, predominantly expressed in striated muscles, including cardiac and skeletal muscles. It localizes to the Z-disc of sarcomeres, where it anchors actin filaments, stabilizes contractile apparatus architecture, and facilitates force transmission during muscle contraction. ACTN2 also interacts with ion channels, signaling proteins, and metabolic enzymes, influencing muscle excitability and mechanotransduction.
Antibodies against ACTN2 are widely used in research to study muscle development, disease mechanisms (e.g., cardiomyopathies, muscular dystrophies), and protein-protein interactions. They enable detection of ACTN2 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry. ACTN2 dysregulation has been linked to cardiac arrhythmias, heart failure, and skeletal muscle disorders, making these antibodies valuable for diagnostic and therapeutic research.
Commercially available ACTN2 antibodies vary in host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and epitope specificity. Validation for species reactivity (human, mouse, rat) and application compatibility is essential for experimental accuracy. Recent studies also explore ACTN2's role in cancer cell motility and metastasis, expanding its relevance beyond muscle biology.
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