| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human HAGH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HAGH抗体的3篇代表性文献示例，涵盖其在不同疾病中的研究应用：
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1. **标题**: *"Hydroxyacylglutathione Hydrolase (HAGH) Expression in Diabetic Nephropathy: A Comparative Study Using Immunohistochemistry"*
**作者**: Zhang L, Chen Y, Wang H.
**摘要**: 本研究利用HAGH特异性抗体，通过免疫组化分析糖尿病肾病(DN)患者肾组织样本。结果显示，HAGH在DN患者的肾小球和肾小管中的表达显著上调，且与氧化应激标志物(如MDA)呈正相关，提示HAGH可能通过调节乙二醛代谢参与DN的病理过程。
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2. **标题**: *"Development of a Monoclonal Antibody Against HAGH and Its Application in Colorectal Cancer Biomarker Discovery"*
**作者**: Tanaka K, et al.
**摘要**: 研究团队成功制备了高特异性的小鼠抗人HAGH单克隆抗体，并通过ELISA和Western blot验证其灵敏度。利用该抗体对结直肠癌组织进行检测，发现HAGH在癌组织中的表达显著高于正常组织，且与患者生存率负相关，提示HAGH可能作为潜在的癌症生物标志物。
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3. **标题**: *"HAGH-Mediated Glyoxalase System Dysfunction in Alzheimer’s Disease: Insights from Post-Mortem Brain Analysis"*
**作者**: Smith J, et al.
**摘要**: 通过HAGH抗体对阿尔茨海默病(AD)患者脑组织进行蛋白质印迹分析，发现HAGH蛋白水平较对照组降低，同时伴随甲基乙二醛(MG)积累。研究提出HAGH活性下降可能导致AD中晚期糖基化终产物(AGEs)的形成增加，加剧神经元损伤。
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**说明**：以上文献为模拟示例，实际研究中需通过学术数据库(如PubMed、Web of Science)检索真实文献。建议使用关键词“HAGH antibody”“glyoxalase system”“HAGH disease”等进一步查找。
The HAGH (Hydroxyacylglutathione Hydrolase) antibody is a tool used to study the HAGH enzyme, also known as glyoxalase II (GLO2), a critical component of the glyoxalase system. This system detoxifies methylglyoxal (MG), a reactive byproduct of glycolysis linked to cellular damage, aging, and pathologies like diabetes, neurodegenerative diseases, and cancer. HAGH catalyzes the hydrolysis of S-D-lactoylglutathione to D-lactate and glutathione, completing the conversion of MG into harmless metabolites.
Research on HAGH antibodies focuses on understanding the enzyme's expression, localization, and role in disease. Studies suggest HAGH dysregulation may contribute to oxidative stress-related conditions, cancer progression, and diabetic complications. Antibodies against HAGH enable detection via techniques like Western blotting, immunohistochemistry, and ELISA, aiding in profiling its tissue distribution and quantifying levels in clinical samples.
Structurally, HAGH is a zinc-dependent metalloenzyme encoded by the HAGH gene on human chromosome 16. Its activity is influenced by genetic polymorphisms and post-translational modifications. Recent interest explores HAGH as a potential therapeutic target or biomarker, particularly in cancers where MG accumulation promotes genomic instability. However, its dual role in cytoprotection and metabolic signaling requires further investigation. HAGH antibodies thus serve as vital reagents in elucidating its pathophysiological mechanisms and therapeutic potential.
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