| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FHI; CPN1; CYP11B; P450C11 |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CYP11B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP11B1抗体的3篇示例参考文献(注：文献为示例性虚构，实际引用请核实原文)：
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1. **文献名称**: *"Production and Characterization of a Specific Polyclonal Antibody Against Human CYP11B1 for Adrenal Studies"*
**作者**: Smith A, et al.
**摘要**: 研究报道了一种针对人CYP11B1酶的多克隆抗体的开发与验证。通过免疫兔获得抗体，并经Western blot和免疫组化验证其特异性，确认其不与CYP11B2交叉反应。抗体成功应用于肾上腺组织切片中CYP11B1的定位分析。
2. **文献名称**: *"CYP11B1 Expression in Adrenocortical Tumors: Immunohistochemical Analysis Using a Novel Monoclonal Antibody"*
**作者**: Tanaka K, et al.
**摘要**: 该研究开发了一种单克隆抗体用于检测肾上腺皮质肿瘤中的CYP11B1表达。通过对比正常与肿瘤组织，发现CYP11B1在皮质醇分泌型肿瘤中高表达，且抗体特异性经siRNA敲低实验验证。
3. **文献名称**: *"Role of CYP11B1 in Congenital Adrenal Hyperplasia: Insights from Antibody-Based Protein Quantification"*
**作者**: Müller B, et al.
**摘要**: 研究利用CYP11B1抗体对先天性肾上腺增生症(CAH)患者肾上腺样本进行蛋白定量，发现CYP11B1表达水平与基因突变类型相关，为CAH的分子机制提供了新证据。
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**提示**：实际文献可通过PubMed或Google Scholar以关键词“CYP11B1 antibody”、“CYP11B1 immunohistochemistry”检索，并筛选涉及抗体开发、验证或应用的论文。
CYP11B1. also known as steroid 11β-hydroxylase, is a mitochondrial cytochrome P450 enzyme primarily expressed in the adrenal cortex. It plays a critical role in cortisol biosynthesis by catalyzing the conversion of 11-deoxycortisol to cortisol, a key glucocorticoid involved in stress response and metabolism regulation. Mutations in the CYP11B1 gene cause congenital adrenal hyperplasia (CAH), specifically the 11β-hydroxylase deficiency subtype, leading to cortisol insufficiency, androgen excess, and hypertension due to mineralocorticoid precursor accumulation.
CYP11B1 antibodies are essential tools for studying adrenal steroidogenesis, diagnosing CAH, and researching adrenal disorders. These antibodies detect CYP11B1 protein expression in tissues or cell lines, aiding in immunohistochemistry, Western blotting, or immunofluorescence to localize the enzyme within adrenal zona fasciculata cells. Due to the high sequence homology between CYP11B1 and CYP11B2 (aldosterone synthase), antibody specificity is crucial to avoid cross-reactivity in studies distinguishing glucocorticoid vs. mineralocorticoid pathways.
In autoimmune contexts, anti-CYP11B1 antibodies are rarely reported but may contribute to adrenal dysfunction in polyendocrine syndromes. Commercially available antibodies enable translational research, including drug development targeting cortisol overproduction in Cushing’s syndrome. Proper validation ensures reliability in both clinical diagnostics and experimental models.
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