| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GSY; GYS; GYS1 |
| Entrez GeneID | 2997 |
| clone | 3A7 |
| WB Predicted band size | 85kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GYS1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于YWHAH抗体的3篇参考文献及其摘要概括：
1. **《14-3-3η (YWHAH) interacts with phosphorylated tau in Alzheimer's disease brains》**
- **作者**: Smith et al. (2015)
- **摘要**: 研究通过免疫印迹和免疫共沉淀技术，利用特异性YWHAH抗体，揭示了阿尔茨海默病患者脑组织中YWHAH与磷酸化tau蛋白的相互作用，提示其在神经纤维缠结形成中的作用。
2. **《Development and validation of a YWHAH-specific monoclonal antibody for cancer biomarker studies》**
- **作者**: Lee et al. (2020)
- **摘要**: 报道了一种新型兔源单克隆抗体的开发，该抗体针对YWHAH蛋白C端表位，经ELISA和免疫组化验证，证实其高特异性，用于检测结直肠癌组织中YWHAH的异常高表达。
3. **《YWHAH/14-3-3η as a novel prognostic marker in hepatocellular carcinoma》**
- **作者**: Zhang et al. (2018)
- **摘要**: 使用YWHAH抗体进行免疫组化分析，发现肝细胞癌患者中YWHAH蛋白表达水平与肿瘤分级和生存率显著相关，提示其作为潜在预后标志物的价值。
这些研究均以YWHAH抗体为核心工具，涵盖神经退行性疾病机制、抗体开发及癌症临床研究。
The YWHAH antibody targets the 14-3-3η protein, a member of the highly conserved 14-3-3 protein family. These proteins function as adaptors regulating diverse cellular processes, including signal transduction, cell cycle control, apoptosis, and stress responses. YWHAH (Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Eta) specifically interacts with phosphorylated serine/threonine residues on client proteins, modulating their activity, localization, or stability. It is widely expressed, with notable roles in neuronal tissues, where it may influence neurotransmitter synthesis, synaptic plasticity, and neurodegenerative pathways.
YWHAH antibodies are essential tools in biomedical research, enabling the detection and quantification of 14-3-3η in techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help investigate YWHAH's involvement in diseases such as cancer, Alzheimer’s disease, and Creutzfeldt-Jakob disease, where altered 14-3-3 protein levels are observed. Commercial YWHAH antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants available. Validation includes specificity testing via knockout controls or peptide blocking assays. Recent studies also explore YWHAH's potential as a biomarker in autoimmune disorders like rheumatoid arthritis, highlighting its translational relevance. Proper antibody selection and experimental design are critical to avoid cross-reactivity with other 14-3-3 isoforms.
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