Rabbit Polyclonal KRT12 Antibody

The KRT12 antibody targets keratin 12 (KRT12), a type I intermediate filament protein encoded by the *KRT12* gene. Primarily expressed in the corneal epithelium, KRT12 pairs with KRT3 (keratin 3) to form a structural network essential for maintaining corneal integrity and mechanical resilience. Mutations in *KRT12* are linked to Meesmann epithelial corneal dystrophy (MECD), a rare autosomal dominant disorder characterized by fragile corneal epithelial cells, microcysts, and photophobia. KRT12 antibodies are vital tools in research and diagnostics, enabling detection of KRT12 expression or dysfunction in corneal tissues. In research, these antibodies help study corneal development, epithelial cell differentiation, and disease mechanisms in models like transgenic mice or in vitro systems. Clinically, they aid in diagnosing MECD by identifying abnormal KRT12 distribution or reduced expression in corneal biopsies. Commercial KRT12 antibodies are typically developed in rabbits or mice using immunogenic peptides from conserved regions of the human KRT12 protein. Validation methods include immunohistochemistry, Western blotting, and immunofluorescence to ensure specificity, particularly in distinguishing KRT12 from other keratins. Their application advances understanding of corneal pathologies and supports therapeutic development for epithelial fragility disorders.