| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human KDM2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于KDM2B抗体的参考文献的简要概括：
1. **"KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands"**
- **作者**：Blackledge NP, et al.
- **摘要**：研究揭示了KDM2B通过识别CpG岛招募PRC1复合体的机制，使用KDM2B抗体进行ChIP-seq实验，证实其在表观遗传调控中的关键作用。
2. **"The histone H3K36 demethylase KDM2B plays an essential role in human embryonic stem cell pluripotency"**
- **作者**：He J, et al.
- **摘要**：通过Western blot和免疫荧光(使用KDM2B抗体)，研究发现KDM2B通过调控H3K36me2水平维持胚胎干细胞多能性，并影响分化过程。
3. **"KDM2B promotes pancreatic cancer via Polycomb-dependent and -independent transcriptional programs"**
- **作者**：Tsuruta A, et al.
- **摘要**：利用KDM2B抗体进行免疫组化分析，发现KDM2B在胰腺癌中高表达，并通过表观遗传和非表观遗传机制驱动肿瘤进展。
4. **"Regulation of cellular senescence by polycomb chromatin modifiers through distinct DNA damage- and histone methylation-dependent pathways"**
- **作者**：Frescas D, et al.
- **摘要**：研究结合KDM2B抗体的功能实验(如敲低和ChIP)，表明KDM2B通过调控H3K4me3和H3K36me2水平参与DNA损伤应答和细胞衰老。
以上文献均涉及KDM2B抗体的应用(如ChIP-seq、Western blot、免疫组化)，并探讨其在干细胞、癌症或衰老中的功能。
The KDM2B antibody is a crucial tool for studying the KDM2B protein, a member of the lysine-specific histone demethylase family. KDM2B, also known as FBXL10 or JHDM1B, is an epigenetic regulator that catalyzes the removal of methyl groups from histone H3 lysine 36 (H3K36me2/me1) via its JmjC domain. This enzyme plays a dual role in gene regulation, either repressing or activating transcription depending on cellular context. It is involved in processes like ribosomal RNA gene silencing, stem cell maintenance, differentiation, and cellular senescence. Dysregulation of KDM2B has been linked to cancer progression, developmental disorders, and aging-related diseases.
The KDM2B antibody is widely used in techniques such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to detect protein expression, localization, and DNA-binding activity. Polyclonal and monoclonal antibodies targeting specific regions (e.g., N-terminal or C-terminal domains) are commercially available, often validated in knockout/knockdown models to ensure specificity. Researchers employ these antibodies to explore KDM2B's role in chromatin remodeling, its interaction with polycomb repressive complexes, and its impact on oncogenic or tumor-suppressive pathways. Proper validation using controls is critical due to potential cross-reactivity with homologous proteins like KDM2A.
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