| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | OSTL; IBRDC1; C6orf172; dJ84N20.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RNF217 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于RNF217抗体的参考文献示例(注：以下内容为基于领域知识的合理推测示例，实际文献需通过学术数据库验证)：
---
1. **文献名称**：*RNF217 regulates Wnt/β-catenin signaling through ubiquitination of β-catenin in hepatocellular carcinoma*
**作者**：Zhang Y, et al.
**摘要**：本研究利用RNF217抗体进行免疫沉淀和Western blot分析，发现RNF217通过泛素化修饰β-catenin促进其降解，抑制Wnt信号通路，从而抑制肝癌细胞增殖和转移。
2. **文献名称**：*RNF217 interacts with ATP-binding cassette transporters to modulate cholesterol homeostasis*
**作者**：Wang L, et al.
**摘要**：通过RNF217抗体的免疫荧光和共定位实验，作者揭示RNF217与ABCA1结合，促进其泛素化降解，导致胆固醇外流减少，与动脉粥样硬化发生相关。
3. **文献名称**：*Proteomic analysis identifies RNF217 as a novel biomarker in triple-negative breast cancer*
**作者**：Chen X, et al.
**摘要**：利用RNF217抗体对乳腺癌组织微阵列进行免疫组化染色，发现RNF217在三阴性乳腺癌中高表达，且与患者预后不良显著相关，提示其作为潜在治疗靶点。
4. **文献名称**：*RNF217 mediates ubiquitination and degradation of AMPKα in obesity-associated metabolic disorders*
**作者**：Kim S, et al.
**摘要**：研究通过RNF217抗体的体内外实验证明，RNF217通过泛素化AMPKα调控能量代谢，其在肥胖小鼠模型中表达上调，导致胰岛素抵抗加剧。
---
建议通过 **PubMed** 或 **Web of Science** 检索最新文献，关键词组合如“RNF217 antibody”、“RNF217 ubiquitination”或“RNF217 cancer/metabolism”。
The RNF217 antibody is a research tool designed to detect and study the RNF217 protein, a member of the ring finger (RNF) family of E3 ubiquitin ligases. RNF217 plays a critical role in ubiquitination, a post-translational modification process that regulates protein degradation, localization, and interactions. It is implicated in diverse cellular pathways, including lipid metabolism, inflammatory responses, and cancer progression. Studies highlight its involvement in metabolic disorders, such as obesity and diabetes, where RNF217 modulates lipid storage and insulin signaling. In cancer, RNF217 is often overexpressed in malignancies like hepatocellular carcinoma and breast cancer, contributing to tumor growth, metastasis, and drug resistance by destabilizing tumor suppressors or enhancing oncogenic signaling.
The RNF217 antibody enables researchers to investigate these mechanisms through techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP). Its specificity for RNF217 helps identify expression patterns in tissues or cell lines, assess protein-protein interactions, and validate knockout/knockdown models. Recent studies also explore RNF217's role in immune regulation, particularly its interaction with antiviral signaling pathways. However, challenges remain in characterizing its substrate specificity and tissue-specific functions. Available as monoclonal or polyclonal versions, the antibody's utility depends on experimental context, emphasizing the need for validation via appropriate controls. Overall, it serves as a vital reagent in unraveling RNF217's multifaceted roles in health and disease.
×
