| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NML; KIAA0409 |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human RRP8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3条关于RRP8抗体的虚构参考文献(基于科学研究的常见方向合理构建)：
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1. **文献名称**: *"RRP8 Antibody Characterization in Epigenetic Regulation of DNA Methylation"*
**作者**: Zhang L, et al.
**摘要**: 本研究利用自主研发的RRP8多克隆抗体，通过免疫沉淀和Western blot验证其在人细胞系中的特异性。研究发现RRP8通过与DNMT3A相互作用调控特定基因启动子区的DNA甲基化，抗体成功应用于染色质免疫共沉淀(ChIP)实验，揭示了RRP8在表观遗传沉默中的新机制。
2. **文献名称**: *"Development of a Monoclonal RRP8 Antibody for Cancer Biomarker Studies"*
**作者**: Tanaka K, et al.
**摘要**: 报道一种高亲和力抗RRP8单克隆抗体的开发，经ELISA和免疫组化验证其在结直肠癌组织中的高表达。研究表明，RRP8表达水平与患者预后显著相关，抗体可用于临床样本检测，为癌症诊断提供潜在生物标志物。
3. **文献名称**: *"RRP8 Nuclear Localization and Its Role in Ribosomal RNA Processing"*
**作者**: Müller S, et al.
**摘要**: 通过免疫荧光结合RRP8特异性抗体，证实RRP8定位于细胞核仁区域。敲低实验显示RRP8缺失导致rRNA前体加工异常，抗体在亚细胞定位研究中的应用支持了其在核糖体生物发生中的关键作用。
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注：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science以“RRP8 antibody”或“RRP8 AND (epigenetics/rRNA/cancer)”为关键词检索真实文献。
The RRP8 (Ribosomal RNA Processing 8) antibody is a tool used to study the RRP8 protein, a conserved methyltransferase involved in ribosomal RNA (rRNA) maturation and epigenetic regulation. RRP8. also known as NSUN5. belongs to the S-adenosylmethionine-dependent methyltransferase family and localizes to the nucleolus, where it facilitates rRNA modification, a critical step in ribosome biogenesis. Emerging research highlights its dual role in chromatin remodeling and DNA damage response, linking it to epigenetic mechanisms such as histone H3K4 methylation and heterochromatin organization. Dysregulation of RRP8 has been implicated in cellular stress responses, aging, and diseases like cancer, where altered expression may influence tumor progression or chemoresistance.
RRP8 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to analyze protein expression, subcellular localization, and interaction partners. They help elucidate RRP8's functional dynamics in cell cycle regulation, nucleolar stress pathways, and crosstalk between ribosome biogenesis and epigenetic control. Challenges include ensuring antibody specificity due to conserved domains in methyltransferases and variability in post-translational modifications across cell types. Recent studies also explore RRP8's potential as a biomarker or therapeutic target, particularly in cancers with dysregulated ribosome machinery. Validation using knockout cell lines or orthogonal methods is recommended for experimental reliability.
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