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Rabbit Polyclonal GTF3A Antibody

  • 中文名: GTF3A抗体
  • 别    名: AP2; TFIIIA
货号: IPDX13542
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/300 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

参考文献

以下是3篇关于GTF3A抗体的模拟参考文献(基于领域知识构建,非真实存在):

1. **文献名称**:*"Development and Characterization of a Monoclonal Antibody Specific for Human GTF3A Protein"*

**作者**:Smith JL, et al.

**摘要**:本研究报道了一种新型小鼠单克隆抗体的开发,该抗体特异性识别人源GTF3A蛋白。通过免疫印迹和免疫荧光验证其特异性,证实该抗体可用于检测内源性GTF3A在细胞核内的定位,并应用于研究其在RNA聚合酶III转录调控中的作用。

2. **文献名称**:*"GTF3A Expression Dynamics and Antibody-Based Detection in Zebrafish Embryogenesis"*

**作者**:Chen R, et al.

**摘要**:利用兔多克隆抗体探究斑马鱼胚胎发育过程中GTF3A的表达模式。研究发现GTF3A在早期神经嵴细胞分化中高表达,抗体通过免疫组化验证了其组织特异性分布,提示GTF3A可能在发育调控中具有保守功能。

3. **文献名称**:*"A Novel GTF3A Antibody Reveals Its Role in Ribosomal RNA Processing and Cancer Cell Proliferation"*

**作者**:Johnson M, et al.

**摘要**:通过CRISPR/Cas9基因敲除结合新开发的GTF3A抗体(克隆号:Ab-7F2),发现GTF3A不仅参与RNA聚合酶III转录,还通过与pre-rRNA结合调控核糖体成熟。抗体在乳腺癌细胞系中验证了GTF3A过表达与细胞增殖的相关性。

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注:以上文献为示例性内容,实际引用时需检索真实数据库(如PubMed)获取最新研究。如需真实文献,可补充具体研究背景或应用场景进一步筛选。

背景信息

The GTF3A antibody targets the transcription factor III A (TFIIIA), a key protein involved in RNA polymerase III (Pol III)-mediated transcription. GTF3A, encoded by the GTF3A gene, is essential for the initiation of 5S ribosomal RNA (5S rRNA) synthesis, a critical component of ribosome assembly. TFIIIA binds directly to the internal promoter of 5S rRNA genes through its zinc finger domains, facilitating the recruitment of other Pol III-associated factors, such as TFIIIC and TFIIIB, to form a transcriptionally active complex. This process is tightly regulated and crucial for cellular protein synthesis, growth, and proliferation. Antibodies against GTF3A are widely used in molecular biology research to study Pol III-dependent transcription mechanisms, ribosome biogenesis, and their dysregulation in diseases like cancer or viral infections. They enable techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to analyze TFIIIA expression, localization, and DNA-binding activity. Commercial GTF3A antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers also utilize these antibodies to explore links between Pol III activity, cellular stress responses, and oncogenic pathways, offering insights into potential therapeutic targets for conditions marked by aberrant ribosome production or transcriptional dysfunction.

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