首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血红蛋白酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、RBC裂解物.样本中的HBA1含量。HBA1是人类血红蛋白α链的编码基因，位于16号染色体上，参与氧气运输和气体交换。其研究机制涉及血红蛋白结构、功能及其与疾病的关系，如α地中海贫血。试剂盒检测范围为0.78 μg/mL-50 μg/mL，适用于科研领域中对红细胞破坏程度评估、血液代谢研究以及药物或毒性物质引起的溶血效应分析，如建立贫血动物模型、监测体外溶血实验或探究血液代用品功效等研究场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hba1 ELISA Kit; Hba-a1 ELISA Kit; Hemoglobin subunit alpha-1/2 ELISA Kit; Alpha-1/2-globin ELISA Kit; Hemoglobin alpha-1/2 chain ELISA Kit
缩写：HBA1
Uniprot No.：P01946
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, lysate for RBC.
检测范围：0.78 μg/mL-50 μg/mL
灵敏度：0.39 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 101 | |||||
| Range % | 95-107 | ||||||
| 1:10 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:40 | Average % | 90 | |||||
| Range % | 85-97 | ||||||
回收率：
| The recovery of rat Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| µg/mlOD1OD2Average 500.1690.1760.1725 250.2920.3110.3015 12.50.4860.4830.4845 6.250.8190.7950.807 3.121.3731.3471.36 1.562.0132.0242.0185 0.782.4612.4932.477 02.7212.6522.6865 | |||||||
货期：3-5 working days
×
