首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：人Gremlin-2(GREM2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GREM2含量。Gremlin-2（GREM2）是一种分泌的糖基化蛋白，属于BMP拮抗剂家族，主要表达于代谢相关组织。GREM2通过抑制BMP信号通路，影响脂肪细胞分化和能量代谢，在肥胖和2型糖尿病的治疗中具有潜在应用价值。试剂盒检测范围为6.25 pg/mL-2500 pg/mL，可广泛应用于细胞模型、疾病动物实验及临床前样本分析，为探究GREM2在组织再生、纤维化病理进程或肿瘤微环境调控中的作用提供可靠工具，助力发育生物学、病理机制及药物干预等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP antagonist 2 ELISA Kit; CKTSF1B2 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; Cysteine knot superfamily 1 BMP antagonist 2 ELISA Kit; DAN domain family member 3 ELISA Kit; DAND 3 ELISA Kit; DAND3 ELISA Kit; GREM 2 ELISA Kit; Grem2 ELISA Kit; GREM2_HUMAN ELISA Kit; Gremlin 2 ELISA Kit; Gremlin 2 cysteine knot superfamily homolog ELISA Kit; Gremlin-2 ELISA Kit; Gremlin2 ELISA Kit; PRDC ELISA Kit; Protein related to DAN and cerberus ELISA Kit
缩写：GREM2
Uniprot No.：Q9H772
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-2500 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GREM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-100 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 84-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-101 | |||||
回收率：
| The recovery of human GREM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-93 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 25000.1550.1490.152 4690.3420.3520.347 780.5590.5690.564 221.0010.9630.982 6.251.5431.5101.527 02.1842.0862.135 | ||||||
货期：3-5 working days
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