| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RP78; SA-RhoGEF; p114RhoGEF; P114-RhoGEF |
| WB Predicted band size | 152 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ARHGEF18 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARHGEF18抗体的3篇模拟参考文献示例(基于常见研究场景，可能需要根据实际文献调整)：
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1. **文献名称**: *ARHGEF18 regulates epithelial cell polarity through RhoA signaling*
**作者**: Smith J, et al.
**摘要**: 本研究利用ARHGEF18特异性抗体，通过免疫荧光和Western blot分析，揭示了ARHGEF18在调控上皮细胞极性中的作用，表明其通过激活RhoA通路影响细胞骨架重排。
2. **文献名称**: *Expression profiling of ARHGEF18 in colorectal cancer using immunohistochemistry*
**作者**: Lee H, et al.
**摘要**: 作者开发了一种高特异性的ARHGEF18抗体，并用于结直肠癌组织样本的免疫组化分析，发现ARHGEF18高表达与肿瘤侵袭性及患者预后不良显著相关。
3. **文献名称**: *ARHGEF18 interacts with Par3 to maintain blood-brain barrier integrity*
**作者**: Zhang Y, et al.
**摘要**: 通过免疫共沉淀(使用ARHGEF18抗体)和功能实验，该研究证明ARHGEF18与Par3蛋白互作，参与血脑屏障稳定性的调控，为神经系统疾病机制提供了新见解。
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**注意**：以上文献为模拟示例，实际引用时需通过PubMed、Web of Science等数据库检索真实文献。若需具体文献，建议使用关键词“ARHGEF18 antibody”或“ARHGEF18 + (Western/IF/IHC)”进一步筛选。
ARHGEF18 (Rho/Rac Guanine Nucleotide Exchange Factor 18), also known as p114RhoGEF, is a member of the RhoGEF family that activates Rho GTPases, particularly RhoA and CDC42. by catalyzing the exchange of GDP for GTP. It plays a critical role in regulating cytoskeletal dynamics, cell polarity, and cell-cell junction formation. Structurally, ARHGEF18 contains a Dbl homology (DH) domain and a pleckstrin homology (PH) domain, which are essential for its guanine nucleotide exchange activity and membrane targeting. The protein is highly expressed in epithelial and endothelial cells, where it contributes to maintaining barrier integrity and coordinating cellular responses to extracellular signals, such as those mediated by G protein-coupled receptors (GPCRs).
ARHGEF18 antibodies are immunological tools designed to detect and quantify the expression, localization, and functional states of ARHGEF18 in various biological samples. These antibodies, often developed in hosts like rabbits or mice, are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Research utilizing ARHGEF18 antibodies has revealed its involvement in pathological processes, including cancer progression, inflammatory diseases, and vascular dysfunction. For example, studies suggest ARHGEF18 dysregulation may influence tumor cell invasion by modulating RhoA-driven actomyosin contractility. Additionally, its role in endothelial cell signaling highlights potential implications in hypertension and atherosclerosis. Validated ARHGEF18 antibodies are essential for exploring its molecular mechanisms and therapeutic relevance in disease contexts.
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